The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis.
Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
Anido J et al. TGF-ß Receptor Inhibitors Target the CD44(high)/Id1(high) Glioma-Initiating Cell Population in Human Glioblastoma. Cancer Cell18:655-68 (2010).
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