Anti-CD15 antibody [MMA], prediluted (ab7495)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CD15 antibody [MMA], prediluted
See all CD15 primary antibodies -
Description
Mouse monoclonal [MMA] to CD15, prediluted -
Host species
Mouse -
Specificity
This antibody reacts with a 220 kD protein. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
BALB/C mice were injected with U937 histiocytic cell line.
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Positive control
- Tonsil.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MMA -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab7495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Application notes
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
Cellular localization
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
Alternative names
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
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Protocols
Datasheets and documents
References
This product has been referenced in:
- Anido J et al. TGF-ß Receptor Inhibitors Target the CD44(high)/Id1(high) Glioma-Initiating Cell Population in Human Glioblastoma. Cancer Cell 18:655-68 (2010). IF ; Human . Read more (PubMed: 21156287) »
