Anti-CD15 antibody [MMA], prediluted (ab7495)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-CD15 antibody [MMA], prediluted
See all CD15 primary antibodies -
DescriptionMouse monoclonal [MMA] to CD15, prediluted
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Host speciesMouse
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SpecificityThis antibody reacts with a 220 kD protein.
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Tested applicationsSuitable for: IHC-P, IHC-Frmore details
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Species reactivityReacts with: Human
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Immunogen
BALB/C mice were injected with U937 histiocytic cell line.
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Positive control
- Tonsil.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituents: PBS
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberMMA
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IsotypeIgM
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab7495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | ||
| IHC-Fr |
Application notes
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
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Cellular localizationGolgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
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Database links
- Entrez Gene: 2526 Human
- Omim: 104230 Human
- SwissProt: P22083 Human
- Unigene: 390420 Human
- Unigene: 572064 Human
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Alternative names
- 3-FAL antibody
- Alpha-3-fucosyltransferase antibody
- ELAM 1 ligand antibody
see all
Protocols
References
This product has been referenced in:
- Anido J et al. TGF-ß Receptor Inhibitors Target the CD44(high)/Id1(high) Glioma-Initiating Cell Population in Human Glioblastoma. Cancer Cell 18:655-68 (2010). IF ; Human . Read more (PubMed: 21156287) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"