Key features and details
- Mouse monoclonal [MMA] to CD15, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgM
Product nameAnti-CD15 antibody [MMA], prediluted
See all CD15 primary antibodies
DescriptionMouse monoclonal [MMA] to CD15, prediluted
SpecificityThis antibody reacts with a 220 kD protein.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human CD15. U937 histiocytic cell line.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Concentration information loading...
PurityProtein A purified
Light chain typekappa
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
Cellular localizationGolgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
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ab7495 has been referenced in 1 publication.
- Anido J et al. TGF-ß Receptor Inhibitors Target the CD44(high)/Id1(high) Glioma-Initiating Cell Population in Human Glioblastoma. Cancer Cell 18:655-68 (2010). IF ; Human . PubMed: 21156287