Anti-CD15 antibody [P12] (ab3358)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-CD15 antibody [P12]
See all CD15 primary antibodies -
DescriptionMouse monoclonal [P12] to CD15
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Host speciesMouse
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Tested applicationsSuitable for: IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Fresh (non-cultured) placenta cell preparation (Human).
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Positive control
- Human kidney tissue.
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General notes
Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. This antibody belongs to the BG-7 group and detects the Lewis x (type 2 chain) blood-group antigen, which is expressed in gastrointestinal tumors, and lung cancers. It is found in crypt cells, which represent the proliferative compartment of the colonic mucosa, and strong homogeneous immunostaining is observed in most metastases and invasive cancer cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituents: 0.0268% PBS, 1% BSA
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PurityTissue culture supernatant
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Primary antibody notesBlood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. This antibody belongs to the BG-7 group and detects the Lewis x (type 2 chain) blood-group antigen, which is expressed in gastrointestinal tumors, and lung cancers. It is found in crypt cells, which represent the proliferative compartment of the colonic mucosa, and strong homogeneous immunostaining is observed in most metastases and invasive cancer cells.
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ClonalityMonoclonal
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Clone numberP12
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IsotypeIgM
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab3358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/40. | |
| ICC/IF | 1/200 - 1/400. |
Target
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RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
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Cellular localizationGolgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
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Database links
- Entrez Gene: 2526 Human
- Omim: 104230 Human
- SwissProt: P22083 Human
- Unigene: 390420 Human
- Unigene: 572064 Human
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Alternative names
- 3-FAL antibody
- Alpha-3-fucosyltransferase antibody
- ELAM 1 ligand antibody
see all
References
This product has been referenced in:
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). Read more (PubMed: 29343722) »
- Shi CS et al. Lectin-like domain of thrombomodulin binds to its specific ligand Lewis Y antigen and neutralizes lipopolysaccharide-induced inflammatory response. Blood 112:3661-70 (2008). Read more (PubMed: 18711002) »
