Overview

  • Product name
    Anti-CD15 antibody [P12]
    See all CD15 primary antibodies
  • Description
    Mouse monoclonal [P12] to CD15
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fresh (non-cultured) placenta cell preparation (Human).

  • Positive control
    • Human kidney tissue.
  • General notes


    Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. This antibody belongs to the BG-7 group and detects the Lewis x (type 2 chain) blood-group antigen, which is expressed in gastrointestinal tumors, and lung cancers. It is found in crypt cells, which represent the proliferative compartment of the colonic mucosa, and strong homogeneous immunostaining is observed in most metastases and invasive cancer cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab3358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/40.
ICC/IF 1/200 - 1/400.

Target

  • Relevance
    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization
    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

References

This product has been referenced in:
  • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). Read more (PubMed: 29343722) »
  • Shi CS  et al. Lectin-like domain of thrombomodulin binds to its specific ligand Lewis Y antigen and neutralizes lipopolysaccharide-induced inflammatory response. Blood 112:3661-70 (2008). Read more (PubMed: 18711002) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your inquiry.

I can confirm that the storage temperature of our products can be found on the individual datasheets.

Short term storage is usually two weeks. If the products is not used within two weeks of purchase, we in general recommend to aliquot it and to store as stated for the long term storage. As a general rule, freeze/thaw cycle are to be avoided.

Please find below the long term storage conditions for the products you are interested in:

ab9100 : Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

ab3358 : Store at +4°C.

ab3359 : Store at +4°C.

ab30667 : Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

ab86128 : Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Since the vials are small and we want the text to be readable, we strongly suggest to check the datasheets for the storage information. This will have the positive side effect that also all the other valuable information on the datasheet will be seen.

I hope this information is helpful and wish you a sunny day.

Read More

Answer

Thank you for your enquiry.

I can confirm that ab3358Blood Group Lewis x antibody [P12] is sold as tissue culture supernatant. I have requested that the datasheetisupdated so thisinformation is clearer. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay,and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for this particular product, theconcentration is estimated at 10-50ug/ml.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your enquiry. This antibody is tissue culture supernatant concentrated by ammonium sulfate precipitation. The concentration of our last lot was 180 ug/ml. We don't currently have any stock of this antibody, so the concentration may change with new stock. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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