Overview

  • Product name

    Anti-CD15 antibody [SP159]
    See all CD15 primary antibodies
  • Description

    Rabbit monoclonal [SP159] to CD15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type corresponding to Human CD15. CD15 purified from U937 cells.

  • Positive control

    • IHC-P: Human Hodgkin’s Lymphoma, spleen, cervix carcinoma, and Mouse kidney tissues FC: THP-1 cells
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab135377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20 - 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50.

Perform heat mediated antigen retrieval by boiling tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with primary antibody for 10 minutes at room temperature.

Target

  • Relevance

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names

    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on proximal tubular epithelial cells in the mouse kidney, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab135377 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervix carcinoma tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human cervix carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab135377 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human Hodgkin's lymphoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab135377 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab135377 for 30 mins at room temperature.

  • Flow cytometry analysis of THP-1 (human acute monocytic leukemia) labeling CD15 with purified ab135377 at 1/20 dilution (7.85 µg/ml) (red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin’s Lymphoma tissue labelling CD15 with ab135377 at 1/100 dilution.

  • Flow cytometric analysis of rabbit anti-CD15 (SP159) antibody  ab135377 (1/200) in HL-60 cells(green) compared to negative control of rabbit IgG (blue).

References

This product has been referenced in:

  • Katsushima K  et al. Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment. Nat Commun 7:13616 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27922002) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Airway tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Permeabilization
No
Specification
Airway tissue
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 27 2016

Answer

Thank you for contacting us.

I can confirm that both ab20137 and ab135377 recognise the carbohydrate CD15 (3-fucosyl-N-acetyl-lactosamine).

ab20137's immunogen is Lewis-X from monocytes, separated from other peripheral blood leucocytes (PBL) on fibronectin plates. Please not that this clone does not activate complement.

ab135377's immunogen is Lewis-X purified from U937 cells.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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