Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993)

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Overview

  • Product name
    Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)]
    See all CD15 primary antibodies
  • Description
    Mouse monoclonal [(Workshop IV) (BRA-4F1) our designation 238)] to CD15
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Erythroid myeloid leukemia cells line K562.

  • Positive control
    • Reed Sternberg cells in Hodgkin’s lymphoma tissue.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 15th June 2017.  Lot numbers higher than GR177792-4 will be from tissue culture supernatant.  Please note that the dilutions may need to be adjusted accordingly.

Properties

Applications

Our Abpromise guarantee covers the use of ab115993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/200. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC Use at an assay dependent concentration.

(methanol fixed) Use at an assay dependent concentration
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization
    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Database links
    • Alternative names
      • 3-FAL antibody
      • Alpha-3-fucosyltransferase antibody
      • ELAM 1 ligand antibody
      • ELAM ligand fucosyltransferase antibody
      • ELAM-1 ligand fucosyltransferase antibody
      • ELFT antibody
      • FCT3A antibody
      • FUC-TIV antibody
      • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) antibody
      • Fucosyltransferase antibody
      • fucosyltransferase IV antibody
      • fucT-IV antibody
      • FUTIV antibody
      • galactoside 3-L-fucosyltransferase antibody
      • Leu M1 antibody
      • Lewis X antibody
      • LeX antibody
      • SSEA-1 antibody
      • stage-specific embryonic antigen 1 antibody
      see all

    References

    ab115993 has not yet been referenced specifically in any publications.

    Publishing research using ab115993? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    Dear Sir/Madam Please kindly recommend the abcam slide control for IHC-P application for this antibodies. 1. Anti-ErbB 2 antibody (ab2428) 2. Anti-Progesterone Receptor antibody [SP2] (ab16661) 3. Anti-Estrogen Receptor antibody [SP1] (ab16660) 4. Ant...

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    Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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