Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993)
Key features and details
- Mouse monoclonal [(Workshop IV) (BRA-4F1) our designation 238)] to CD15
- Suitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IF
- Reacts with: Species independent
- Isotype: IgM
Overview
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Product name
Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)]
See all CD15 primary antibodies -
Description
Mouse monoclonal [(Workshop IV) (BRA-4F1) our designation 238)] to CD15 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Erythroid myeloid leukemia cells line K562.
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Positive control
- Reed Sternberg cells in Hodgkin’s lymphoma tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 15th June 2017. Lot numbers higher than GR177792-4 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
(Workshop IV) (BRA-4F1) our designation 238) -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab115993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/200. Predicted molecular weight: 59 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | 1/50 - 1/100. | |
| ICC | Use at an assay dependent concentration. (methanol fixed) Use at an assay dependent concentration |
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| ICC/IF | Use at an assay dependent concentration. |
Target
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Relevance
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
Cellular localization
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
Alternative names
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
Protocols
References (0)
ab115993 has not yet been referenced specifically in any publications.
