Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)]
See all CD15 primary antibodies -
Description
Mouse monoclonal [(Workshop IV) (BRA-4F1) our designation 238)] to CD15 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Erythroid myeloid leukemia cells line K562.
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Positive control
- Reed Sternberg cells in Hodgkin’s lymphoma tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 15th June 2017. Lot numbers higher than GR177792-4 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 98% PBS, 1% BSA -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
(Workshop IV) (BRA-4F1) our designation 238) -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab115993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/200. Predicted molecular weight: 59 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | 1/50 - 1/100. | |
| ICC | Use at an assay dependent concentration. (methanol fixed) Use at an assay dependent concentration |
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| ICC/IF | Use at an assay dependent concentration. |
Target
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Relevance
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
Cellular localization
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
Alternative names
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
Protocols
References
ab115993 has not yet been referenced specifically in any publications.
