Key features and details
- Mouse monoclonal [11G5a] to CD151
- Suitable for: WB, Flow Cyt, ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD151 antibody [11G5a]
See all CD151 primary antibodies
DescriptionMouse monoclonal [11G5a] to CD151
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, ELISAmore details
Species reactivityReacts with: Human
The details of the immunogen for this antibody are not available.
- Tonsil/spleen. WB: HT1080 whole cell lysate. ICC/IF: Human fibrosarcoma cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab33315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.|
|Flow Cyt||1/50 - 1/100.
1/50 - 1/100. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
|ICC/IF||Use at an assay dependent concentration. Use at a concentration of 10 µg/ml for 1 hr.|
|ELISA||Use at an assay dependent concentration.|
FunctionEssential for the proper assembly of the glomerular and tubular basement membranes in kidney.
Tissue specificityExpressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.
Involvement in diseaseDefects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD) [MIM:609057]. NPEBD is characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
- Information by UniProt
- CD151 antibody
- CD151 antigen antibody
- CD151 molecule (Raph blood group) antibody
ab33315 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and blocked with 100% Image-iTTM signal enhancer for 30 minutes at 20°C. The primary antibody was used at 10µg/ml concentration and incubated with sample for 1 hour at 25°C. Alexa Fluor®555 conjugated donkey monoclonal to mouse (H+L) was used as the secondary antibody, diluted 1/500.
Anti-CD151 antibody [11G5a] (ab33315) at 1/1000 dilution + HT1080 (human fibrosarcoma cell line) whole cell lysate
Sheep anti-mouse IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
ICC/IF image of ab33315 stained HT 1080 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33315, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96789, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab33315 has been referenced in 10 publications.
- Jiang L et al. Expression of tetraspanins NET-6 and CD151 in breast cancer as a potential tumor biomarker. Clin Exp Med N/A:N/A (2019). PubMed: 31004251
- Shikanai M et al. Caveolin-1 Promotes Early Neuronal Maturation via Caveolae-Independent Trafficking of N-Cadherin and L1. iScience 7:53-67 (2018). PubMed: 30267686
- Donnenberg VS et al. Antibody-based cell-surface proteome profiling of metastatic breast cancer primary explants and cell lines. Cytometry A 93:448-457 (2018). PubMed: 29498809
- Zhao Y et al. MiR-124 aggravates failing hearts by suppressing CD151-facilitated angiogenesis in heart. Oncotarget 9:14382-14396 (2018). PubMed: 29581851
- Zeng P et al. Tetraspanin CD151 as an emerging potential poor prognostic factor across solid tumors: a systematic review and meta-analysis. Oncotarget 8:5592-5602 (2017). PubMed: 27888619
- Palmer TD et al. Integrin-free tetraspanin CD151 can inhibit tumor cell motility upon clustering and is a clinical indicator of prostate cancer progression. Cancer Res 74:173-87 (2014). PubMed: 24220242
- Matsumoto N et al. Role of CD151 expression in gallbladder carcinoma. Surgery 156:1212-7 (2014). IHC-P ; Human . PubMed: 24890568
- Sandvig K & Llorente A Proteomic analysis of microvesicles released by the human prostate cancer cell line PC-3. Mol Cell Proteomics 11:M111.012914 (2012). WB ; Human . PubMed: 22457534
- Zhang Z et al. Comparative proteomic analysis of plasma membrane proteins between human osteosarcoma and normal osteoblastic cell lines. BMC Cancer 10:206 (2010). IHC-P ; Human . PubMed: 20470422
- Karamatic Crew V et al. CD151, the first member of the tetraspanin (TM4) superfamily detected on erythrocytes, is essential for the correct assembly of human basement membranes in kidney and skin. Blood 104:2217-23 (2004). PubMed: 15265795