Anti-CD16 antibody [3G8] - Low endotoxin, Azide free (ab176528)
Key features and details
- Mouse monoclonal [3G8] to CD16 - Low endotoxin, Azide free
- Suitable for: IP, Functional Studies, IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD16 antibody [3G8] - Low endotoxin, Azide free
See all CD16 primary antibodies -
Description
Mouse monoclonal [3G8] to CD16 - Low endotoxin, Azide free -
Host species
Mouse -
Tested applications
Suitable for: IP, Functional Studies, IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Tissue, cells or virus corresponding to Human CD16. Human neutrophils.
Database link: P08637 -
General notes
Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab176528 is purified from hybridoma culture supernatant by protein-A affinity chromatography. Purity: > 95% (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
3G8 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PE Anti-CD16 antibody [3G8] (ab117117)
- PerCP Anti-CD16 antibody [3G8] (ab130761)
- PerCP/Cy5.5® Anti-CD16 antibody [3G8], prediluted (ab157322)
- Alexa Fluor® 488 Anti-CD16 antibody [3G8] (ab187585)
- APC/Cy7® Anti-CD16 antibody [3G8], prediluted (ab197141)
- PE/Cy7® Anti-CD16 antibody [3G8] (ab234209)
- FITC Anti-CD16 antibody [3G8] (ab239254)
- Alexa Fluor® 647 Anti-CD16 antibody [3G8] (ab239275)
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
Functional Studies |
Use at an assay dependent concentration.
In vitro Stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion. |
|
IHC-Fr |
Use at an assay dependent concentration.
Acetone fixation. |
|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IP
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. In vitro Stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion. |
IHC-Fr
Use at an assay dependent concentration. Acetone fixation. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. -
Tissue specificity
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage. -
Cellular localization
Cell membrane. Secreted. Exists also as a soluble receptor. - Information by UniProt
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Database links
- Entrez Gene: 2214 Human
- Omim: 146740 Human
- SwissProt: P08637 Human
- Unigene: 372679 Human
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Alternative names
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab176528 has not yet been referenced specifically in any publications.