Product nameAnti-CD16 antibody [3G8] - Low endotoxin, Azide free
See all CD16 primary antibodies
DescriptionMouse monoclonal [3G8] to CD16 - Low endotoxin, Azide free
Tested applicationsSuitable for: Functional Studies, IHC-Fr, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Non human primates
Tissue, cells or virus corresponding to Human CD16. Human neutrophils.
Database link: P08637
Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein A purified
Purification notesab176528 is purified from hybridoma culture supernatant by protein-A affinity chromatography. Purity: > 95% (by SDS-PAGE).
- Anti-CD16 antibody [3G8] (Phycoerythrin) (ab117117)
- Anti-CD16 antibody [3G8] (PerCP) (ab130761)
- Anti-CD16 antibody [3G8], prediluted (PerCP/Cy5.5®) (ab157322)
- Anti-CD16 antibody [3G8] (Alexa Fluor® 488) (ab187585)
- Anti-CD16 antibody [3G8], prediluted (Allophycocyanin/Cy7 ®) (ab197141)
- Anti-CD16 antibody [3G8] (PE/Cy7 ®) (ab234209)
- Anti-CD16 antibody [3G8] (FITC) (ab239254)
- Anti-CD16 antibody [3G8] (Alexa Fluor® 647) (ab239275)
- Anti-CD16 antibody [3G8] (PE/Cy7 ®) (ab267386)
Our Abpromise guarantee covers the use of ab176528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
In vitro Stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion
|IHC-Fr||Use at an assay dependent concentration.
|Flow Cyt||Use a concentration of 6 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
FunctionReceptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Tissue specificityExpressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage.
Cellular localizationCell membrane. Secreted. Exists also as a soluble receptor.
- Information by UniProt
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
ab176528 has not yet been referenced specifically in any publications.