Key features and details
- Mouse monoclonal [3G8] to CD16 (PE/Cy7®)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG1
Product nameAnti-CD16 antibody [3G8] (PE/Cy7®)
See all CD16 primary antibodies
DescriptionMouse monoclonal [3G8] to CD16 (PE/Cy7®)
ConjugationPE/Cy7®. Ex: 496nm, Em: 774nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
The details of the immunogen for this antibody are not available.
- Flow Cyt: Human peripheral blood lymphocytes.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
Concentration information loading...
Purification notesThe purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Light chain typekappa
- Anti-CD16 antibody [3G8] (PE) (ab117117)
- Anti-CD16 antibody [3G8] (PerCP) (ab130761)
- Anti-CD16 antibody [3G8], prediluted (PerCP/Cy5.5®) (ab157322)
- Anti-CD16 antibody [3G8] - Low endotoxin, Azide free (ab176528)
- Anti-CD16 antibody [3G8] (Alexa Fluor® 488) (ab187585)
- Anti-CD16 antibody [3G8], prediluted (APC/Cy7®) (ab197141)
- Anti-CD16 antibody [3G8] (PE/Cy7®) (ab234209)
- Anti-CD16 antibody [3G8] (FITC) (ab239254)
- Anti-CD16 antibody [3G8] (Alexa Fluor® 647) (ab239275)
Our Abpromise guarantee covers the use of ab267386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
The antibody has been diluted for use at 5 µL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µL. The number of cells within a sample should be determined empirically, but typically ranges between 1x105 to 1x108 cells.
FunctionReceptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Tissue specificityExpressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage.
Cellular localizationCell membrane. Secreted. Exists also as a soluble receptor.
- Information by UniProt
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab267386 has not yet been referenced specifically in any publications.