Key features and details
- Rabbit polyclonal to CD16
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CD16 antibody
See all CD16 primary antibodies
DescriptionRabbit polyclonal to CD16
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- U937 cells; Human lung cancer, human glioma and mouse spleen tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Aqueous buffered solution
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/20 - 1/100.|
|IHC-P||1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200.
|WB||1/100 - 1/1000. Predicted molecular weight: 29 kDa.|
FunctionReceptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Tissue specificityExpressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage.
Cellular localizationCell membrane. Secreted. Exists also as a soluble receptor.
- Information by UniProt
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
U937 cells probed with ab203883 at a 1/100 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG PE conjugated secondary at 1/100 (green) for 40 minutes compared to control cells (blue).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human glioma tissue labeling CD16 with ab203883 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung cancer tissue labeling CD16 with ab203883 at 1/200 dilution followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue labeling CD16 with ab203883 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
ab203883 has been referenced in 8 publications.
- Zhou J et al. LncGBP9/miR-34a axis drives macrophages toward a phenotype conducive for spinal cord injury repair via STAT1/STAT6 and SOCS3. J Neuroinflammation 17:134 (2020). PubMed: 32345320
- Meng Q et al. Protective effects of histone deacetylase inhibition by Scriptaid on brain injury in neonatal rat models of cerebral ischemia and hypoxia. Int J Clin Exp Pathol 13:179-191 (2020). PubMed: 32211098
- Xu M et al. Hollow mesoporous ruthenium nanoparticles conjugated bispecific antibody for targeted anti-colorectal cancer response of combination therapy. Nanoscale 11:9661-9678 (2019). PubMed: 31065660
- Wang L et al. Borneol alleviates brain injury in sepsis mice by blocking neuronal effect of endotoxin. Life Sci 232:116647 (2019). PubMed: 31301416
- Akhmetzyanova ER et al. Transplantation of Microglia in the Area of Spinal Cord Injury in an Acute Period Increases Tissue Sparing, but Not Functional Recovery. Front Cell Neurosci 12:507 (2018). PubMed: 30631265
- Pasquier J et al. Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction. Sci Rep 7:16450 (2017). PubMed: 29180664
- An Q et al. Clinical significance of lymphocyte subset changes in hemophagocytic lymphohistiocytosis of children. Exp Ther Med 12:3549-3552 (2016). PubMed: 28105088
- Chvialeva KI et al. [Clinico-experimental study of hormonal changes in penetrating eye injuries]. Oftalmol Zh 32:603-5 (1977). WB ; Mouse . PubMed: 203883