Recombinant Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16784] to CD16 - Low endotoxin, Azide free
- Suitable for: WB, Mass Cytometry, IHC-P
- Reacts with: Rat, Human, Recombinant fragment
Overview
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Product name
Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free
See all CD16 primary antibodies -
Description
Rabbit monoclonal [EPR16784] to CD16 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P RatHumanMass Cytometry Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human liver and thymus, Rat liver tissues. IMC: Human tonsil tissue
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General notes
ab215977 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16784 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IHC-P |
Rat
Human
|
Mass Cytometry |
Recombinant fragment
|
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration.
|
|
Mass Cytometry | (1) |
Use at an assay dependent concentration.
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use at an assay dependent concentration. |
Mass Cytometry
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. -
Tissue specificity
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage. -
Cellular localization
Cell membrane. Secreted. Exists also as a soluble receptor. - Information by UniProt
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Database links
- Entrez Gene: 2214 Human
- Entrez Gene: 304966 Rat
- Omim: 146740 Human
- SwissProt: P08637 Human
- SwissProt: P27645 Rat
- Unigene: 372679 Human
- Unigene: 23977 Rat
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Alternative names
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
see all
Images
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Mass Cytometry - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University
Imaging Mass Cytometry™ (IMC™) image of human tonsil tissue stained with Anti-CD16 antibody [EPR16784]. ab215977 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.
Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on kupffer cell of rat liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Using PBS instead of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198507).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)
Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on macrophage of Human thymus tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Using PBS instead of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198507).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on kupffer cell of Human liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Using PBS instead of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198507).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Datasheets and documents
Certificate of Compliance
References (0)
ab215977 has not yet been referenced specifically in any publications.