Recombinant
RabMAb

Recombinant Anti-CD16 antibody [EPR20537] (ab211151)

Overview

  • Product name
    Anti-CD16 antibody [EPR20537]
    See all CD16 primary antibodies
  • Description
    Rabbit monoclonal [EPR20537] to CD16
  • Host species
    Rabbit
  • Specificity
    We recommend ICC for human only.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human CD16 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P08637

  • Positive control
    • WB: Human fetal brain lysate; Rat brain and P0 retina lysates; C6, U-937, THP-1, Jurkat, K-562 and PC-12 whole cell lysates. ICC/IF: Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 29 kDa).
ICC/IF 1/100.

Target

  • Function
    Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
  • Tissue specificity
    Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Glycosylated. Contains high mannose- and complex-type oligosaccharides.
    The soluble form is produced by a proteolytic cleavage.
  • Cellular localization
    Cell membrane. Secreted. Exists also as a soluble receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 16 antibody
    • CD 16a antibody
    • CD16 antibody
    • CD16a antibody
    • CD16a antigen antibody
    • CD16B antibody
    • CD16b antigen antibody
    • Fc fragment of IgG antibody
    • Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG receptor IIIa antibody
    • Fc fragment of IgG, low affinity III, receptor (CD16) antibody
    • Fc fragment of IgG, low affinity III, receptor for (CD16) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor for antibody
    • Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody
    • Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody
    • Fc gamma R3 antibody
    • Fc gamma receptor III 2 (CD 16) antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIA antibody
    • Fc gamma receptor IIIb (CD 16) antibody
    • Fc gamma RIII alpha antibody
    • Fc gamma RIII antibody
    • Fc gamma RIII beta antibody
    • Fc gamma RIIIa antibody
    • Fc gamma RIIIb antibody
    • Fc of IgG antibody
    • Fc-gamma receptor III2 (CD 16) antibody
    • Fc-gamma receptor III2 (CD16) antibody
    • Fc-gamma receptor IIIb (CD16) antibody
    • Fc-gamma RIII antibody
    • Fc-gamma RIII-alpha antibody
    • Fc-gamma RIIIa antibody
    • FCG 3 antibody
    • FCG3 antibody
    • FCG3A_HUMAN antibody
    • FCgammaRIIIA antibody
    • FCGR 3 antibody
    • FCGR 3A antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3A protein antibody
    • FCGRIII antibody
    • FCGRIII-2 antibody
    • FcR 10 antibody
    • FcR-10 antibody
    • FcR10 antibody
    • FcRIII antibody
    • FcRIIIa antibody
    • IGFR 3 antibody
    • IGFR3 antibody
    • IgG Fc receptor III 1 antibody
    • IgG Fc receptor III 2 antibody
    • IgG Fc receptor III-2 antibody
    • IMD20 antibody
    • immunoglobulin G Fc receptor III antibody
    • immunoglobulin G Fc receptor III-2 antibody
    • Low affinity IIIa receptor antibody
    • Low affinity immunoglobulin gamma Fc region receptor III A antibody
    • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
    • Low affinity immunoglobulin gamma Fc region receptor IIIB antibody
    • neutrophil-specific antigen NA antibody
    see all

Images

  • All lanes : Anti-CD16 antibody [EPR20537] (ab211151) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 (rat glial tumor cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lane 1: 26 seconds; Lane 2: 70 seconds; Lane 3: 3 minutes.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28652325).

  • All lanes : Anti-CD16 antibody [EPR20537] (ab211151) at 1/1000 dilution

    Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
    Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 6 : Rat P0 retina lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa



    Exposure time : Lanes 1 and 2: 3 minutes; Lanes 3-6: 37 seconds.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28652325).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD16 with ab211151 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Jurkat cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

References

ab211151 has not yet been referenced specifically in any publications.

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