Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)

Overview

  • Product name
    Anti-CD16 antibody [EPR22409-124] - BSA and Azide free
    See all CD16 primary antibodies
  • Description
    Rabbit monoclonal [EPR22409-124] to CD16 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD16 aa 1-200. The exact sequence is proprietary.
    Database link: O75015

  • Positive control
    • WB: Human spleen and colon cancer lysates. IP: Human spleen lysate. IHC-P: Human spleen and liver tissue. ICC/IF: Human PBMCs. Flow cyt: Human PBMCs.
  • General notes

    ab252908 is a PBS-only buffer format of ab246222. Please refer to ab246222 for recommended dilutions, protocols, and image data.

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab252908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 50-70 kDa (predicted molecular weight: 29 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
  • Tissue specificity
    Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Glycosylated. Contains high mannose- and complex-type oligosaccharides.
    The soluble form is produced by a proteolytic cleavage.
  • Cellular localization
    Cell membrane. Secreted. Exists also as a soluble receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 16 antibody
    • CD 16a antibody
    • CD16 antibody
    • CD16a antibody
    • CD16a antigen antibody
    • CD16B antibody
    • CD16b antigen antibody
    • Fc fragment of IgG antibody
    • Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG receptor IIIa antibody
    • Fc fragment of IgG, low affinity III, receptor (CD16) antibody
    • Fc fragment of IgG, low affinity III, receptor for (CD16) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor for antibody
    • Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody
    • Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody
    • Fc gamma R3 antibody
    • Fc gamma receptor III 2 (CD 16) antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIA antibody
    • Fc gamma receptor IIIb (CD 16) antibody
    • Fc gamma RIII alpha antibody
    • Fc gamma RIII antibody
    • Fc gamma RIII beta antibody
    • Fc gamma RIIIa antibody
    • Fc gamma RIIIb antibody
    • Fc of IgG antibody
    • Fc-gamma receptor III2 (CD 16) antibody
    • Fc-gamma receptor III2 (CD16) antibody
    • Fc-gamma receptor IIIb (CD16) antibody
    • Fc-gamma RIII antibody
    • Fc-gamma RIII-alpha antibody
    • Fc-gamma RIIIa antibody
    • FCG 3 antibody
    • FCG3 antibody
    • FCG3A_HUMAN antibody
    • FCgammaRIIIA antibody
    • FCGR 3 antibody
    • FCGR 3A antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3A protein antibody
    • FCGRIII antibody
    • FCGRIII-2 antibody
    • FcR 10 antibody
    • FcR-10 antibody
    • FcR10 antibody
    • FcRIII antibody
    • FcRIIIa antibody
    • IGFR 3 antibody
    • IGFR3 antibody
    • IgG Fc receptor III 1 antibody
    • IgG Fc receptor III 2 antibody
    • IgG Fc receptor III-2 antibody
    • IMD20 antibody
    • immunoglobulin G Fc receptor III antibody
    • immunoglobulin G Fc receptor III-2 antibody
    • Low affinity IIIa receptor antibody
    • Low affinity immunoglobulin gamma Fc region receptor III A antibody
    • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
    • Low affinity immunoglobulin gamma Fc region receptor IIIB antibody
    • neutrophil-specific antigen NA antibody
    see all

Images

  • CD16 was immunoprecipitated from 0.35 mg of human spleen lysate with ab246222 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab246222 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human spleen lysate 10 μg (Input). 

    Lane 2: ab246222 IP in human spleen lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246222 in human spleen lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Human PBMCs (human peripheral blood mononuclear cells) were stained with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)(Left) or ab246222 (Right) followed by a Goat anti rabbit IgG (Dylight ® 488) at 1/2000 dilution. They were then stained with Alexa Fluor® 647-conjugated anti-CD14. The expression pattern is consistent with what described in the literature. (PMID: 21738687)

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Negative control: Raji. (PMID:11207281).

    Raji (human Burkitt's lymphoma cell line) were stained with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)(Left) or ab246222 (Right) followed by a Goat anti rabbit IgG (Dylight ® 488) at 1/2000 dilution.  

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD16 with ab246222 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kuffer cells of human liver (PMID: 26512139) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab246222 for 10 minutes at 37?. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD16 with ab246222 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red pulp of human spleen (PMID: 26512139; 29692344). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab246222 for 10 minutes at 37ºC. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMCs (human peripheral blood mononuclear cells) or Raji (human Burkitt's lymphoma cell line) cell line labeling CD16 with ab246222 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of human PBMC cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: Raji (PMID:11207281).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

References

ab252908 has not yet been referenced specifically in any publications.

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