Overview

  • Product name
    Anti-CD16 antibody [MEM-154] - Azide free
    See all CD16 primary antibodies
  • Description
    Mouse monoclonal [MEM-154] to CD16 - Azide free
  • Host species
    Mouse
  • Specificity
    The antibody MEM-154 reacts with the epitope on CD16 antigen residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcγRIII antigen). MEM-154 recognizes a polymorphism of CD16a (NK cells form), it reacts well with granulocyte form (CD16b) of all subjects. The antibody blocks binding of human IgG to FcγRIII.
  • Tested applications
    Suitable for: WB, IP, Functional Studies, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human granulocytes

  • Positive control
    • Peripheral blood lymphocytes
  • General notes
    Preserved by filter sterilization

Properties

Applications

Our Abpromise guarantee covers the use of ab46679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Functional Studies
Flow Cyt
IHC-Fr
  • Application notes
    Flow Cyt: Use at a concentration of 5 - 10 µg/ml.
    FuncS: Use at an assay dependent dilution. The antibody blocks binding of human IgG to FcγRIII.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Use under non reducing conditions. Predicted molecular weight: 29 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
    • Tissue specificity
      Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
    • Sequence similarities
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Glycosylated. Contains high mannose- and complex-type oligosaccharides.
      The soluble form is produced by a proteolytic cleavage.
    • Cellular localization
      Cell membrane. Secreted. Exists also as a soluble receptor.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 16 antibody
      • CD 16a antibody
      • CD16 antibody
      • CD16a antibody
      • CD16a antigen antibody
      • CD16B antibody
      • CD16b antigen antibody
      • Fc fragment of IgG antibody
      • Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
      • Fc fragment of IgG low affinity IIIa receptor antibody
      • Fc fragment of IgG receptor IIIa antibody
      • Fc fragment of IgG, low affinity III, receptor (CD16) antibody
      • Fc fragment of IgG, low affinity III, receptor for (CD16) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor for antibody
      • Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody
      • Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody
      • Fc gamma R3 antibody
      • Fc gamma receptor III 2 (CD 16) antibody
      • Fc gamma receptor III A antibody
      • Fc gamma receptor IIIA antibody
      • Fc gamma receptor IIIb (CD 16) antibody
      • Fc gamma RIII alpha antibody
      • Fc gamma RIII antibody
      • Fc gamma RIII beta antibody
      • Fc gamma RIIIa antibody
      • Fc gamma RIIIb antibody
      • Fc of IgG antibody
      • Fc-gamma receptor III2 (CD 16) antibody
      • Fc-gamma receptor III2 (CD16) antibody
      • Fc-gamma receptor IIIb (CD16) antibody
      • Fc-gamma RIII antibody
      • Fc-gamma RIII-alpha antibody
      • Fc-gamma RIIIa antibody
      • FCG 3 antibody
      • FCG3 antibody
      • FCG3A_HUMAN antibody
      • FCgammaRIIIA antibody
      • FCGR 3 antibody
      • FCGR 3A antibody
      • FCGR3 antibody
      • FCGR3A antibody
      • FCGR3A protein antibody
      • FCGRIII antibody
      • FCGRIII-2 antibody
      • FcR 10 antibody
      • FcR-10 antibody
      • FcR10 antibody
      • FcRIII antibody
      • FcRIIIa antibody
      • IGFR 3 antibody
      • IGFR3 antibody
      • IgG Fc receptor III 1 antibody
      • IgG Fc receptor III 2 antibody
      • IgG Fc receptor III-2 antibody
      • IMD20 antibody
      • immunoglobulin G Fc receptor III antibody
      • immunoglobulin G Fc receptor III-2 antibody
      • Low affinity IIIa receptor antibody
      • Low affinity immunoglobulin gamma Fc region receptor III A antibody
      • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
      • Low affinity immunoglobulin gamma Fc region receptor IIIB antibody
      • neutrophil-specific antigen NA antibody
      see all

    Images

    • Human peripheral blood granulocytes stained with ab46679 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab46679, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood granulocytes.

    References

    This product has been referenced in:
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us. The antibody ab46679 blocks binding of human IgG to FcgammaRIII. The experiment was published in: Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. 

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    Answer

    Thank you for your enquiry. I can confirm the following details which I have obtained from the laboratories: ab41899 CD32 antibody: Unfortunately, we do not have a specific protocol for the use of this product in blocking experiments, this application is supported based upon the literature. There are a number of articles which may be helpful to you, I have listed some examples below: Larsson M, Berge J, Johansson AG, Forsum U. Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro. Immunology. 1997 Jan;90(1):138-46. PMID: 9038724 Bredius RG, Fijen CA, De Haas M, Kuijper EJ, Weening RS, Van de Winkel JG, Out TA. Role of neutrophil Fc gamma RIIa (CD32) and Fc gamma RIIIb (CD16) polymorphic forms in phagocytosis of human IgG1- and IgG3-opsonized bacteria and erythrocytes. Immunology. 1994 Dec;83(4):624-30. PMID: 7875742 Pawar P, Shin PK, Mousa SA, Ross JM, Konstantopoulos K. Fluid shear regulates the kinetics and receptor specificity of Staphylococcus aureus binding to activated platelets. J Immunol. 2004 Jul 15;173(2):1258-65. PMID: 15240718 ab46679 CD16 antibody This antibody can be used for blocking IgG binding to FcγRIII. Further details can be found in the following references: HLDA IV - PART 4: NK13.3 section - NK cell structural studies: Localization of the epitopes of hu CD16 MAbs on the molecular model of CD16, Tamm, A., et al., p. 324-326. Tamm A, Schmidt RE.: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81. PMID: 8759741 I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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