Recombinant
RabMAb

Recombinant Anti-CD16 antibody [SP175] - BSA and Azide free (ab243925)

Overview

  • Product name
    Anti-CD16 antibody [SP175] - BSA and Azide free
    See all CD16 primary antibodies
  • Description
    Rabbit monoclonal [SP175] to CD16 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD16 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P08637

  • Positive control
    • IHC-P: Human lung, thymus, B cell lymphoma, uterus, spleen, placenta, liver, liver hepatocellular carcinoma, lung adenocarcinoma, pancreatic adenocarcinoma and HK lymphoma tissues. Flow Cyt: Jurkat cells.
  • General notes

    ab243925 is a PBS-only buffer format of ab183354. Please refer to ab183354 for recommended dilutions, protocols, and image data.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab243925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Flow Cyt Use at an assay dependent concentration.

Target

  • Function
    Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
  • Tissue specificity
    Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Glycosylated. Contains high mannose- and complex-type oligosaccharides.
    The soluble form is produced by a proteolytic cleavage.
  • Cellular localization
    Cell membrane. Secreted. Exists also as a soluble receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 16 antibody
    • CD 16a antibody
    • CD16 antibody
    • CD16a antibody
    • CD16a antigen antibody
    • CD16B antibody
    • CD16b antigen antibody
    • Fc fragment of IgG antibody
    • Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG receptor IIIa antibody
    • Fc fragment of IgG, low affinity III, receptor (CD16) antibody
    • Fc fragment of IgG, low affinity III, receptor for (CD16) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody
    • Fc fragment of IgG, low affinity IIIa, receptor for antibody
    • Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody
    • Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody
    • Fc gamma R3 antibody
    • Fc gamma receptor III 2 (CD 16) antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIA antibody
    • Fc gamma receptor IIIb (CD 16) antibody
    • Fc gamma RIII alpha antibody
    • Fc gamma RIII antibody
    • Fc gamma RIII beta antibody
    • Fc gamma RIIIa antibody
    • Fc gamma RIIIb antibody
    • Fc of IgG antibody
    • Fc-gamma receptor III2 (CD 16) antibody
    • Fc-gamma receptor III2 (CD16) antibody
    • Fc-gamma receptor IIIb (CD16) antibody
    • Fc-gamma RIII antibody
    • Fc-gamma RIII-alpha antibody
    • Fc-gamma RIIIa antibody
    • FCG 3 antibody
    • FCG3 antibody
    • FCG3A_HUMAN antibody
    • FCgammaRIIIA antibody
    • FCGR 3 antibody
    • FCGR 3A antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3A protein antibody
    • FCGRIII antibody
    • FCGRIII-2 antibody
    • FcR 10 antibody
    • FcR-10 antibody
    • FcR10 antibody
    • FcRIII antibody
    • FcRIIIa antibody
    • IGFR 3 antibody
    • IGFR3 antibody
    • IgG Fc receptor III 1 antibody
    • IgG Fc receptor III 2 antibody
    • IgG Fc receptor III-2 antibody
    • IMD20 antibody
    • immunoglobulin G Fc receptor III antibody
    • immunoglobulin G Fc receptor III-2 antibody
    • Low affinity IIIa receptor antibody
    • Low affinity immunoglobulin gamma Fc region receptor III A antibody
    • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
    • Low affinity immunoglobulin gamma Fc region receptor IIIB antibody
    • neutrophil-specific antigen NA antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human HK lymphoma tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling CD16 with ab183354 at a 1/100 dilution (green) compared to a negative control Rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Immunohistochemical analysis of paraffin embedded human thymus tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Immunohistochemical analysis of paraffin embedded human B cell lymphoma tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Immunohistochemical analysis of paraffin embedded human lung tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Immunohistochemical analysis of paraffin embedded human uterus tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Immunohistochemical analysis of paraffin embedded human spleen tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Immunohistochemical analysis of paraffin embedded human liver tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Immunohistochemical analysis of paraffin embedded human liver hepatocellular carcinoma tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Immunohistochemical analysis of paraffin embedded human lung adenocarcinoma tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

  • Immunohistochemical analysis of paraffin embedded human pancreatic adenocarcinoma tissue labeling CD16 with ab183354 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183354).

References

ab243925 has not yet been referenced specifically in any publications.

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