Anti-CD161 antibody [4C6A11] (ab233785)
Key features and details
- Mouse monoclonal [4C6A11] to CD161
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-CD161 antibody [4C6A11]
See all CD161 primary antibodies -
Description
Mouse monoclonal [4C6A11] to CD161 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD161 aa 50 to the C-terminus. (Expressed in E.coli).
Database link: Q12918 -
Positive control
- WB: Recombinant human CD161 (AA: extra 67-225) protein and CD161 (AA: extra 67-225)-hIgGFc transfected HEK-293 whole cell lysate. Flow Cyt: Raji cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4C6A11 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/200 - 1/400.
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WB |
1/500 - 1/2000.
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Notes |
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Flow Cyt
1/200 - 1/400. |
WB
1/500 - 1/2000. |
Target
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Function
Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. -
Tissue specificity
Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN-glycosylated. Contains sialic acid residues. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3820 Human
- Omim: 602890 Human
- SwissProt: Q12918 Human
- Unigene: 169824 Human
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Alternative names
- C-type lectin domain family 5 member B antibody
- CD161 antibody
- CLEC5B antibody
see all
Images
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Anti-CD161 antibody [4C6A11] (ab233785) at 1/500 dilution + Recombinant human CD161 (AA: extra 67-225) protein
Developed using the ECL technique.Expected MW is 48.4 kDa.
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All lanes : Anti-CD161 antibody [4C6A11] (ab233785) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : CD161 (AA: extra 67-225)-hIgGFc transfected HEK-293 whole cell lysate
Developed using the ECL technique. -
Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cells labeling CD161 with ab233785 at 1/200 dilution (green) compared with a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233785 has not yet been referenced specifically in any publications.