Key features and details
- Mouse monoclonal [B199.2] to CD161
- Suitable for: Flow Cyt, IHC-Fr, IP
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD161 antibody [B199.2]
See all CD161 primary antibodies
DescriptionMouse monoclonal [B199.2] to CD161
Specificityab23624 recognises CD161 cell surface antigen, expressed by almost all NK cells and with a small subset of CD3 positive T cells.
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Activated human NK cells.
EpitopeClone B199.2 recognises a similar epitope to the DX9 monoclonal antibody.
General notesMyeloma cell line: P2X63.Ag8.653
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab23624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells.
Use 10µl for 106 cells in 100µl, or 1/50 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
FunctionPlays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
Tissue specificityExpressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
Sequence similaritiesContains 1 C-type lectin domain.
modificationsN-glycosylated. Contains sialic acid residues.
- Information by UniProt
- C-type lectin domain family 5 member B antibody
- CD161 antibody
- CLEC5B antibody
ab23624 has been referenced in 2 publications.
- Llibre A et al. LLT1 and CD161 Expression in Human Germinal Centers Promotes B Cell Activation and CXCR4 Downregulation. J Immunol 196:2085-94 (2016). IHC . PubMed: 26829983
- Barnig C et al. Lipoxin a4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma. Sci Transl Med 5:174ra26 (2013). IHC-P ; Human . PubMed: 23447017