Overview

  • Product name

    Anti-CD161 antibody [EP7169]
    See all CD161 primary antibodies
  • Description

    Rabbit monoclonal [EP7169] to CD161
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminal of Human CD161 (UniProt ID: Q12918).

  • Positive control

    • Human small intestine tissue lysate, HuT-78, THP1, and Caco 2 cell lysates; HuT-78 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP7169
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 25 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
    • Tissue specificity

      Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
    • Sequence similarities

      Contains 1 C-type lectin domain.
    • Post-translational
      modifications

      N-glycosylated. Contains sialic acid residues.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • C-type lectin domain family 5 member B antibody
      • CD161 antibody
      • CLEC5B antibody
      • HNKR-P1a antibody
      • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
      • Killer cell lectin-like receptor subfamily B member 1 antibody
      • KLRB1 antibody
      • KLRB1_HUMAN antibody
      • Natural killer cell surface protein P1A antibody
      • NKR antibody
      • NKR P1 antibody
      • NKR-P1A antibody
      • NKRP1 antibody
      • NKRP1A antibody
      see all

    Images

    • Flow cytometric analysis of HuT-78 cells labelling CD161 with ab137059 at 1/10 dilution (red) or a Rabbit IgG negative control(green).
    • All lanes : Anti-CD161 antibody [EP7169] (ab137059) at 1/1000 dilution

      Lane 1 : Human small intestine tissue lysate
      Lane 2 : HuT-78 cell lysate
      Lane 3 : THP1 cell lysate
      Lane 4 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 25 kDa
      Observed band size: 44 kDa
      why is the actual band size different from the predicted?

    References

    ab137059 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137059.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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