Key features and details
- Rabbit monoclonal [EP7169] to CD161
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CD161 antibody [EP7169]
See all CD161 primary antibodies
DescriptionRabbit monoclonal [EP7169] to CD161
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues on the C terminal of Human CD161 (UniProt ID: Q12918).
- WB: Human small intestine tissue lysate, HuT-78, THP1, and Caco 2 cell lysates. Flow Cyt: HuT-78 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 25 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPlays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
Tissue specificityExpressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
Sequence similaritiesContains 1 C-type lectin domain.
modificationsN-glycosylated. Contains sialic acid residues.
- Information by UniProt
- C-type lectin domain family 5 member B antibody
- CD161 antibody
- CLEC5B antibody
Flow cytometric analysis of HuT-78 cells labelling CD161 with ab137059 at 1/10 dilution (red) or a Rabbit IgG negative control (green). FITC-conugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a 1/150 dilution.
All lanes : Anti-CD161 antibody [EP7169] (ab137059) at 1/1000 dilution
Lane 1 : Human small intestine tissue lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : THP1 cell lysate
Lane 4 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
ab137059 has not yet been referenced specifically in any publications.