Recombinant

Recombinant Anti-CD161 antibody [EPR21236] (ab234107)

Overview

  • Product name

    Anti-CD161 antibody [EPR21236]
    See all CD161 primary antibodies
  • Description

    Rabbit monoclonal [EPR21236] to CD161
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Mouse CD161 aa 50-250 (extracellular).
    Database link: P27811

  • Positive control

    • IHC-P: FFPE mouse spleen tissue sections.
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21236
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab234107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.01 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
  • Tissue specificity

    Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Post-translational
    modifications

    N-glycosylated. Contains sialic acid residues.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-type lectin domain family 5 member B antibody
    • CD161 antibody
    • CLEC5B antibody
    • HNKR-P1a antibody
    • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
    • Killer cell lectin-like receptor subfamily B member 1 antibody
    • KLRB1 antibody
    • KLRB1_HUMAN antibody
    • Natural killer cell surface protein P1A antibody
    • NKR antibody
    • NKR P1 antibody
    • NKR-P1A antibody
    • NKRP1 antibody
    • NKRP1A antibody
    see all

Images

  • IHC image of CD161 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BONDTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab234107, 0.01µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab234107 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 Sodium Citrate
Permeabilization
No
Specification
Spleen
Fixative
Formaldehyde

Marne Prinsloo

Verified customer

Submitted Dec 18 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up