Overview

  • Product name

    Anti-CD161 antibody [IMG14F1F11]
    See all CD161 primary antibodies
  • Description

    Mouse monoclonal [IMG14F1F11] to CD161
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Amino acids 68-225 of Human CD161 (NP_002249.1).

  • Positive control

    • Recombinant CD161 protein; Human kidney tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    IMG14F1F11
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.

Target

  • Function

    Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
  • Tissue specificity

    Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Post-translational
    modifications

    N-glycosylated. Contains sialic acid residues.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-type lectin domain family 5 member B antibody
    • CD161 antibody
    • CLEC5B antibody
    • HNKR-P1a antibody
    • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
    • Killer cell lectin-like receptor subfamily B member 1 antibody
    • KLRB1 antibody
    • KLRB1_HUMAN antibody
    • Natural killer cell surface protein P1A antibody
    • NKR antibody
    • NKR P1 antibody
    • NKR-P1A antibody
    • NKRP1 antibody
    • NKRP1A antibody
    see all

Images

  • Anti-CD161 antibody [IMG14F1F11] (ab138702) at 1 µg/ml + Recombinant CD161 protein

    Predicted band size: 25 kDa

References

ab138702 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us in Glasgow.

The HIERprotocol used for obtaining the lovely image was the following:

Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes.
Remove from heat and let stand at room temperature in buffer - 20 minutes.
Rinse in 1X TBS with Tween (TBST) – 1 minute @ RT.

Please have in mind that the results may need a bit of optimization.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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