Overview

  • Product name
    Anti-CD161 antibody [PK136] (PE/Cy7 ®)
    See all CD161 primary antibodies
  • Description
    Mouse monoclonal [PK136] to CD161 (PE/Cy7 ®)
  • Host species
    Mouse
  • Conjugation
    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Flow Cyt: C57Bl/6 splenocytes.
  • General notes

    The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of CD161 (NK1.1).

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
  • Clonality
    Monoclonal
  • Clone number
    PK136
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Use 0.5 µg.

Target

  • Function
    Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
  • Tissue specificity
    Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Post-translational
    modifications
    N-glycosylated. Contains sialic acid residues.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-type lectin domain family 5 member B antibody
    • CD161 antibody
    • CLEC5B antibody
    • HNKR-P1a antibody
    • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
    • Killer cell lectin-like receptor subfamily B member 1 antibody
    • KLRB1 antibody
    • KLRB1_HUMAN antibody
    • Natural killer cell surface protein P1A antibody
    • NKR antibody
    • NKR P1 antibody
    • NKR-P1A antibody
    • NKRP1 antibody
    • NKRP1A antibody
    see all

Images

  • Flow cytometric analysis of  C57Bl/6 splenocytes stained with APC Anti-Mouse CD3e and 0.5 µg ab241100 (right panel) or 0.5 µg PE-Cyanine7 Mouse IgG2a isotype control (left panel).

References

ab241100 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241100.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

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