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  1. Link

    cd163-antibody-10d6-ab201461.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-CD163 antibody [10D6] (ab201461)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (5)

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Key features and details

  • Mouse monoclonal [10D6] to CD163
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD163 antibody [10D6]
    See all CD163 primary antibodies
  • Description

    Mouse monoclonal [10D6] to CD163
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant protein encoding of domains 1-4 of the N-terminal region of human CD163.

  • Epitope

    Domains 1 to 4 of the N-terminal region
  • Positive control

    • Human placenta tissue.
  • General notes

    This is the concentrated version of ab74604.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    10D6
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Innate and adaptive immunity

Associated products

  • Alternative Versions

    • Anti-CD163 antibody [10D6], prediluted (ab74604)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human CD163 protein (ab112384)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab201461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
    After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
  • Tissue specificity

    Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
  • Sequence similarities

    Contains 9 SRCR domains.
  • Domain

    The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
  • Post-translational
    modifications

    A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
    Phosphorylated.
  • Cellular localization

    Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
  • Target information above from: UniProt accession Q86VB7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9332 Human
    • Omim: 605545 Human
    • SwissProt: Q86VB7 Human
    • Unigene: 504641 Human
    • Alternative names

      • C163A_HUMAN antibody
      • CD 163 antibody
      • CD163 antibody
      • CD163 antigen antibody
      • CD163 molecule antibody
      • Hemoglobin scavenger receptor antibody
      • M130 antibody
      • M130 antigen precursor antibody
      • Macrophage associated antigen antibody
      • MM130 antibody
      • OTTHUMP00000238617 antibody
      • OTTHUMP00000238618 antibody
      • OTTHUMP00000238619 antibody
      • OTTHUMP00000238620 antibody
      • SCARI1 antibody
      • Scavenger receptor cysteine rich type 1 protein M130 antibody
      • sCD163 antibody
      • Soluble CD163 antibody
      see all

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab201461? Please let us know so that we can cite the reference in this datasheet.

    ab201461 has been referenced in 5 publications.

    • Moreira D  et al. Myeloid cell-targeted STAT3 inhibition sensitizes head and neck cancers to radiotherapy and T cell-mediated immunity. J Clin Invest 131:N/A (2021). PubMed: 33232304
    • Peterson MS  et al. Plasmodium vivax Parasite Load Is Associated With Histopathology in Saimiri boliviensis With Findings Comparable to P vivax Pathogenesis in Humans. Open Forum Infect Dis 6:ofz021 (2019). PubMed: 30937329
    • Kim GW  et al. Hepatitis C Virus Core Protein Promotes miR-122 Destabilization by Inhibiting GLD-2. PLoS Pathog 12:e1005714 (2016). PubMed: 27366906
    • Chen SJ  et al. Distribution and clinical significance of tumour-associated macrophages in pancreatic ductal adenocarcinoma: a retrospective analysis in China. Curr Oncol 22:e11-9 (2015). PubMed: 25684992
    • Osterholm C  et al. Increased expression of heparanase in symptomatic carotid atherosclerosis. Atherosclerosis 226:67-73 (2013). PubMed: 23137827

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD163 antibody [10D6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted May 20 2021

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