Key features and details
- Mouse monoclonal [10D6] to CD163
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD163 antibody [10D6]
See all CD163 primary antibodies
DescriptionMouse monoclonal [10D6] to CD163
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant protein encoding of domains 1-4 of the N-terminal region of human CD163.
EpitopeDomains 1 to 4 of the N-terminal region
- Human placenta tissue.
This is the concentrated version of ab74604.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab201461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/50.|
FunctionAcute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Tissue specificityExpressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
Sequence similaritiesContains 9 SRCR domains.
DomainThe SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
modificationsA soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Cellular localizationSecreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
- Information by UniProt
- C163A_HUMAN antibody
- CD 163 antibody
- CD163 antibody
ab201461 has been referenced in 4 publications.
- Peterson MS et al. Plasmodium vivax Parasite Load Is Associated With Histopathology in Saimiri boliviensis With Findings Comparable to P vivax Pathogenesis in Humans. Open Forum Infect Dis 6:ofz021 (2019). PubMed: 30937329
- Kim GW et al. Hepatitis C Virus Core Protein Promotes miR-122 Destabilization by Inhibiting GLD-2. PLoS Pathog 12:e1005714 (2016). PubMed: 27366906
- Chen SJ et al. Distribution and clinical significance of tumour-associated macrophages in pancreatic ductal adenocarcinoma: a retrospective analysis in China. Curr Oncol 22:e11-9 (2015). PubMed: 25684992
- Osterholm C et al. Increased expression of heparanase in symptomatic carotid atherosclerosis. Atherosclerosis 226:67-73 (2013). PubMed: 23137827