Overview

  • Product name

    Anti-CD163 antibody [EPR14336]
    See all CD163 primary antibodies
  • Description

    Rabbit monoclonal [EPR14336] to CD163
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD163 aa 100-200. The exact sequence is proprietary.
    Database link: Q86VB7

  • Positive control

    • WB: Human spleen, fetal liver and placenta lysate. IHC-P: Human liver and thymus tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 125 kDa).

Target

  • Function

    Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
    After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
  • Tissue specificity

    Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
  • Sequence similarities

    Contains 9 SRCR domains.
  • Domain

    The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
  • Post-translational
    modifications

    A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
    Phosphorylated.
  • Cellular localization

    Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • C163A_HUMAN antibody
    • CD 163 antibody
    • CD163 antibody
    • CD163 antigen antibody
    • CD163 molecule antibody
    • Hemoglobin scavenger receptor antibody
    • M130 antibody
    • M130 antigen precursor antibody
    • Macrophage associated antigen antibody
    • MM130 antibody
    • OTTHUMP00000238617 antibody
    • OTTHUMP00000238618 antibody
    • OTTHUMP00000238619 antibody
    • OTTHUMP00000238620 antibody
    • SCARI1 antibody
    • Scavenger receptor cysteine rich type 1 protein M130 antibody
    • sCD163 antibody
    • Soluble CD163 antibody
    see all

Images

  • All lanes : Anti-CD163 antibody [EPR14336] (ab199427) at 1/2000 dilution

    Lane 1 : Human spleen lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human placenta lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 125 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD163 with ab199427 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Kupffer cells staining in liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used only secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling CD163 with ab199427 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on human thymus tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used only secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab199427 has not yet been referenced specifically in any publications.

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