Recombinant Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
- Datasheet
- References (8)
- Protocols
Overview
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Product name
Anti-CD163 antibody [EPR14643-36] - C-terminal
See all CD163 primary antibodies -
Description
Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CD163 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- Human placenta and Human fetal liver lysates; Human liver and tonsil tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14643-36 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab189915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/30 - 1/40. | |
| WB | 1/1000 - 1/10000. | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval using EDTA based pH 9.0 buffer. |
Target
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Function
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. -
Tissue specificity
Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. -
Sequence similarities
Contains 9 SRCR domains. -
Domain
The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes. -
Post-translational
modificationsA soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Phosphorylated. -
Cellular localization
Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells. - Information by UniProt
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Database links
- Entrez Gene: 9332 Human
- Omim: 605545 Human
- SwissProt: Q86VB7 Human
- Unigene: 504641 Human
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Alternative names
- C163A_HUMAN antibody
- CD 163 antibody
- CD163 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-225787-anti-cd163-antibody-epr14643-36-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD163 with ab189915 at 1/1000 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
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![Immunoprecipitation - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-225792-anti-cd163-antibody-epr14643-36-c-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)Immunoprecipitation. ab189915 at 1/1000 labeling CD163 in Human fetal kidney lysate immunoprecipitated using ab189915 at 1/50.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-338967-anti-cd163-antibody-epr14643-36-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)Immunohistochemical analysis of paraffin embedded human liver tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed.Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-338965-anti-cd163-antibody-epr14643-36-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.
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![Western blot - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-225786-anti-cd163-antibody-epr14643-36-c-terminal-western-blot.jpg)
Western blot - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)All lanes : Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915) at 1/5000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Datasheets and documents
References
This product has been referenced in:
- Maidji E et al. Cellular HIV Reservoirs and Viral Rebound from the Lymphoid Compartments of 4'-Ethynyl-2-Fluoro-2'-Deoxyadenosine (EFdA)-Suppressed Humanized Mice. Viruses 11:N/A (2019). Read more (PubMed: 30871222) »
- Sun PP et al. Urine macrophages reflect kidney macrophage content during acute tubular interstitial and glomerular injury. Clin Immunol 205:65-74 (2019). Read more (PubMed: 31212026) »
