Recombinant Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-225787-anti-cd163-antibody-epr14643-36-c-terminal-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD163 antibody [EPR14643-36] - C-terminal
See all CD163 primary antibodies -
Description
Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CD163 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- Human placenta and Human fetal liver lysates; Human liver and tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14643-36 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab189915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/30 - 1/40. | |
| WB | 1/1000 - 1/10000. | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval using EDTA based pH 9.0 buffer. |
Target
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Function
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. -
Tissue specificity
Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. -
Sequence similarities
Contains 9 SRCR domains. -
Domain
The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes. -
Post-translational
modificationsA soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Phosphorylated. -
Cellular localization
Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells. - Information by UniProt
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Database links
- Entrez Gene: 9332 Human
- Omim: 605545 Human
- SwissProt: Q86VB7 Human
- Unigene: 504641 Human
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Alternative names
- C163A_HUMAN antibody
- CD 163 antibody
- CD163 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD163 with ab189915 at 1/1000 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
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![Immunoprecipitation - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-225792-anti-cd163-antibody-epr14643-36-c-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)Immunoprecipitation. ab189915 at 1/1000 labeling CD163 in Human fetal kidney lysate immunoprecipitated using ab189915 at 1/50.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-338967-anti-cd163-antibody-epr14643-36-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)Immunohistochemical analysis of paraffin embedded human liver tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed.Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-338965-anti-cd163-antibody-epr14643-36-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.
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![Western blot - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-225786-anti-cd163-antibody-epr14643-36-c-terminal-western-blot.jpg)
Western blot - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)All lanes : Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915) at 1/5000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution -
![Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)](https://www.abcam.com/ps/products/189/ab189915/Images/ab189915-7-benefits-of-recombinant-antibodies.png)
Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
Protocols
Datasheets and documents
Certificate of Compliance
References (11)
ab189915 has been referenced in 11 publications.
- Scheffel J et al. Cold-induced urticarial autoinflammatory syndrome related to factor XII activation. Nat Commun 11:179 (2020). PubMed: 31924766
- Wang C et al. Integration of intratumoral RASSF10 expression and tumor-associated macrophages into the established clinical indicators better predicts the prognosis of clear cell renal cell carcinoma patients. Oncoimmunology 9:1736793 (2020). PubMed: 32313718
- Yu P et al. CD163?SRCR5 MARC-145 Cells Resist PRRSV-2 Infection via Inhibiting Virus Uncoating, Which Requires the Interaction of CD163 With Calpain 1. Front Microbiol 10:3115 (2019). PubMed: 32038556
- Maidji E et al. Cellular HIV Reservoirs and Viral Rebound from the Lymphoid Compartments of 4'-Ethynyl-2-Fluoro-2'-Deoxyadenosine (EFdA)-Suppressed Humanized Mice. Viruses 11:N/A (2019). PubMed: 30871222
- Sun PP et al. Urine macrophages reflect kidney macrophage content during acute tubular interstitial and glomerular injury. Clin Immunol 205:65-74 (2019). PubMed: 31212026
