For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    cd163-antibody-epr14643-36-c-terminal-ab189915.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email
RecombinantRabMAb

Recombinant Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)

  • Datasheet
  • Certificate of Compliance
Reviews (2) Submit a question References (11)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
  • Immunoprecipitation - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
  • Western blot - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
  • Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human CD163 protein (ab112384)
Primary
Product image
Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150] (ab203992)
Primary
Product image
Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)

View more associated products

Overview

  • Product name

    Anti-CD163 antibody [EPR14643-36] - C-terminal
    See all CD163 primary antibodies
  • Description

    Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD163 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human placenta and Human fetal liver lysates; Human liver and tonsil tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14643-36
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Innate and adaptive immunity

Associated products

  • Alternative Versions

    • Anti-CD163 antibody [EPR14643-36] - Low endotoxin, Azide free (ab215976)
    • Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)
    • Alexa Fluor® 647 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218294)
    • Biotin Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218295)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD163 protein (ab112384)

Applications

Our Abpromise guarantee covers the use of ab189915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30 - 1/40.
WB 1/1000 - 1/10000.
IHC-P 1/500 - 1/1000.

Perform heat mediated antigen retrieval using EDTA based pH 9.0 buffer.

  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
      After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
    • Tissue specificity

      Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
    • Sequence similarities

      Contains 9 SRCR domains.
    • Domain

      The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
    • Post-translational
      modifications

      A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
      Phosphorylated.
    • Cellular localization

      Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
    • Target information above from: UniProt accession Q86VB7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9332 Human
      • Omim: 605545 Human
      • SwissProt: Q86VB7 Human
      • Unigene: 504641 Human
      • Alternative names

        • C163A_HUMAN antibody
        • CD 163 antibody
        • CD163 antibody
        • CD163 antigen antibody
        • CD163 molecule antibody
        • Hemoglobin scavenger receptor antibody
        • M130 antibody
        • M130 antigen precursor antibody
        • Macrophage associated antigen antibody
        • MM130 antibody
        • OTTHUMP00000238617 antibody
        • OTTHUMP00000238618 antibody
        • OTTHUMP00000238619 antibody
        • OTTHUMP00000238620 antibody
        • SCARI1 antibody
        • Scavenger receptor cysteine rich type 1 protein M130 antibody
        • sCD163 antibody
        • Soluble CD163 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)

        Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD163 with ab189915 at 1/1000 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

      • Immunoprecipitation - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
        Immunoprecipitation - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)

        Immunoprecipitation. ab189915 at 1/1000 labeling CD163 in Human fetal kidney lysate immunoprecipitated using ab189915 at 1/50.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)

        Immunohistochemical analysis of paraffin embedded human liver tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed.Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)

        Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.

      • Western blot - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
        Western blot - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
        All lanes : Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915) at 1/5000 dilution

        Lane 1 : Human placenta lysate
        Lane 2 : Human fetal liver lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
      • Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)
        Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (11)

      Publishing research using ab189915? Please let us know so that we can cite the reference in this datasheet.

      ab189915 has been referenced in 11 publications.

      • Scheffel J  et al. Cold-induced urticarial autoinflammatory syndrome related to factor XII activation. Nat Commun 11:179 (2020). PubMed: 31924766
      • Wang C  et al. Integration of intratumoral RASSF10 expression and tumor-associated macrophages into the established clinical indicators better predicts the prognosis of clear cell renal cell carcinoma patients. Oncoimmunology 9:1736793 (2020). PubMed: 32313718
      • Yu P  et al. CD163?SRCR5 MARC-145 Cells Resist PRRSV-2 Infection via Inhibiting Virus Uncoating, Which Requires the Interaction of CD163 With Calpain 1. Front Microbiol 10:3115 (2019). PubMed: 32038556
      • Maidji E  et al. Cellular HIV Reservoirs and Viral Rebound from the Lymphoid Compartments of 4'-Ethynyl-2-Fluoro-2'-Deoxyadenosine (EFdA)-Suppressed Humanized Mice. Viruses 11:N/A (2019). PubMed: 30871222
      • Sun PP  et al. Urine macrophages reflect kidney macrophage content during acute tubular interstitial and glomerular injury. Clin Immunol 205:65-74 (2019). PubMed: 31212026
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD163 antibody [EPR14643-36] - C-terminal

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Lungs)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: TRS PH.9
      Permeabilization
      No
      Specification
      Lungs
      Blocking step
      (agent) for 12 minute(s) · Concentration: 0% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Syeda Mariam Riaz

      Verified customer

      Submitted Oct 16 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD163 antibody [EPR14643-36] - C-terminal

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BlockAid as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
      Sample
      Human Tissue sections (Skin)
      Specification
      Skin
      Permeabilization
      Yes - 0.2% Triton-X100
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 06 2015

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.