Product nameAnti-CD163 antibody [EPR14643-36] - C-terminal
See all CD163 primary antibodies
DescriptionRabbit monoclonal [EPR14643-36] to CD163 - C-terminal
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Recombinant fragment within Human CD163 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
- Human placenta and Human fetal liver lysates; Human liver and tonsil tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab189915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/30 - 1/40.|
|WB||1/1000 - 1/10000.|
|IHC-P||1/500 - 1/1000.
Perform heat mediated antigen retrieval using EDTA based pH 9.0 buffer.
FunctionAcute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Tissue specificityExpressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
Sequence similaritiesContains 9 SRCR domains.
DomainThe SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
modificationsA soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Cellular localizationSecreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
- Information by UniProt
- C163A_HUMAN antibody
- CD 163 antibody
- CD163 antibody
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD163 with ab189915 at 1/1000 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
Immunoprecipitation. ab189915 at 1/1000 labeling CD163 in Human fetal kidney lysate immunoprecipitated using ab189915 at 1/50.
Immunohistochemical analysis of paraffin embedded human liver tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed.Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.
Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.
All lanes : Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915) at 1/5000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
ab189915 has been referenced in 8 publications.
- Maidji E et al. Cellular HIV Reservoirs and Viral Rebound from the Lymphoid Compartments of 4'-Ethynyl-2-Fluoro-2'-Deoxyadenosine (EFdA)-Suppressed Humanized Mice. Viruses 11:N/A (2019). PubMed: 30871222
- Sun PP et al. Urine macrophages reflect kidney macrophage content during acute tubular interstitial and glomerular injury. Clin Immunol 205:65-74 (2019). PubMed: 31212026
- Tseng HY et al. Oncogenic MCT-1 activation promotes YY1-EGFR-MnSOD signaling and tumor progression. Oncogenesis 6:e313 (2017). IHC ; Human . PubMed: 28394354
- Cheng Z et al. CD163 as a novel target gene of STAT3 is a potential therapeutic target for gastric cancer. Oncotarget 8:87244-87262 (2017). PubMed: 29152078
- Jiang X et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology 6:e1333210 (2017). PubMed: 28811976
- Chen LM et al. Oligo-Fucoidan prevents IL-6 and CCL2 production and cooperates with p53 to suppress ATM signaling and tumor progression. Sci Rep 7:11864 (2017). PubMed: 28928376
- Perelygina L et al. Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies. J Allergy Clin Immunol 138:1436-1439.e11 (2016). IF ; Human . PubMed: 27613149
- Jurado KA et al. Zika virus productively infects primary human placenta-specific macrophages. JCI Insight 1:N/A (2016). IHC-P, ICC/IF ; Human, African green monkey . PubMed: 27595140