The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 125 kDa.
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
Contains 9 SRCR domains.
The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. Phosphorylated.
Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
Immunocytochemistry/ Immunofluorescence - Anti-CD163 antibody [OTI2G12] (ab156769)This image is courtesy of an anonymous Abreview
ab156769 staining CD163 in Human skeletal muscle (Gastrocnemius) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methacarn and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-mouse polyclonal was used as the secondary antibody (1/200).
Immunofluorescent analysis of COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY CD163 labeling CD163 with ab156769 at 1/100 dilution.
Western blot - Anti-CD163 antibody [OTI2G12] (ab156769)
All lanes : Anti-CD163 antibody [OTI2G12] (ab156769) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY CD163 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 125 kDa
HEK-293T cell lysates were generated from transient transfection of the cDNA clone (RC212446)
Tian W et al. A prognostic risk model for patients with triple negative breast cancer based on stromal natural killer cells, tumor-associated macrophages and growth-arrest specific protein 6. Cancer Sci107:882-9 (2016).
Read more (PubMed: 27145494) »
Ha DM et al. Transforming growth factor-beta 1 produced by vascular smooth muscle cells predicts fibrosis in the gastrocnemius of patients with peripheral artery disease. J Transl Med14:39 (2016).
Read more (PubMed: 26847457) »