Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD163 antibody [2G12]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris (pH8) Cit (pH) does not work
Sample
Human Tissue sections (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Permeabilization
No
Fixative
Methacarn

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Antibody Diluent Reagent
Dilution
1/150

Secondary antibody

Name
Non-Abcam antibody was used: Thermo UltraVision Quanto
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
-DAB exposure time should be 1 minute or less -Hydrogen peroxide block helped remove background

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Submitted Jul 29 2013

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