Western blot abreview for Anti-CD163 antibody [2G12]

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/5000

Detection

Detection method
IF
Bands
Specific: 125kDa kDa Non-specific: 27kDa, 37kDa kDa
Exposure
2 minute(s) and 0 second(s)
Positive control
CON = Healthy patient known to express basal levels of macrophages

Additional data

Additional Notes
POS = Patient of interest Non specific bands more faint than band of interest in POS
PubMed ID
GR17970-15: View

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Submitted Aug 26 2013

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