Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD163 antibody [OTI2G12]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin (epidermis to SQ fat or muscle))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Invitrogen eBioscience IHC antigen retrieval solution, low pH, 10x
Permeabilization
Yes - H2O2
Specification
Skin (epidermis to SQ fat or muscle)
Blocking step
ab64226 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
10% neutral buffered saline

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight
Dilution
1/150

Secondary antibody

Name
Non-Abcam antibody was used: Abcam antibody was used but form would not let me
Host species: Goat
Clonality: Polyclonal
Conjugation: Streptavidin

Additional data

Additional Notes
We wanted to see if this would work with swine tissue. It looks like we may be able to use this by tweaking some of our protocol, but we aren't yet trusting it to label all macrophages 100%.
Abcam response
Thank you for participating in the AbTrial testing program and sharing your feedback on use in pig.

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Submitted Feb 15 2018

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