Product nameAnti-CD163 antibody [RM3/1]
See all CD163 primary antibodies
DescriptionMouse monoclonal [RM3/1] to CD163
Tested applicationsSuitable for: WB, Flow Cyt, IP, Functional Studies, ICC/IF, IHC-Frmore details
Unsuitable for: IHC-G or IHC-P
Species reactivityReacts with: Human, Monkey
Does not react with: Rat, Guinea pig, Pig
Human monocytes (epitope recognized SRCR domain 9).
- ICC/IF: M2-type tumor-associated macrophages. Human placenta. CHO-cells transfected with CD163.
ab17051 is very useful for macrophage phenotyping. Positive staining can be seen in the skin (histiocytes), gut, Kupffer cells, few aveolar macrophages, main population of macrophages in the placenta, varying degrees of macrophages in inflammed tissue including tumorous tissue depending on the inflammatory stage. Red pulp, not white pulp, macrophages in the spleen and cortical macrophages of the thymus are detected. Antibody is sensitive to ionic detergents and to chloroform/methanol treatment.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
Purification notes0.2 µm filtered.
Primary antibody notesab17051 is very useful for macrophage phenotyping. Positive staining can be seen in the skin (histiocytes), gut, Kupffer cells, few aveolar macrophages, main population of macrophages in the placenta, varying degrees of macrophages in inflammed tissue including tumorous tissue depending on the inflammatory stage. Red pulp, not white pulp, macrophages in the spleen and cortical macrophages of the thymus are detected. Antibody is sensitive to ionic detergents and to chloroform/methanol treatment.
Our Abpromise guarantee covers the use of ab17051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 130 kDa.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 4 µg/ml. PubMed: 22438948|
FunctionAcute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Tissue specificityExpressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
Sequence similaritiesContains 9 SRCR domains.
DomainThe SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
modificationsA soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Cellular localizationSecreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
- Information by UniProt
- C163A_HUMAN antibody
- CD 163 antibody
- CD163 antibody
Human M2-type tumor-associated macrophages stained for CD163 using ab17051 (green) at 1/200 dilution in IHC (Frozen Sections).
Flow cytometric detection of CD163 in THP-1 cells. The black line represents cells only, the red line the isotype control and the blue line ab17051 in a concentration of 2 μg/250000 cells.
ab17051 has been referenced in 12 publications.
- Li L et al. MiR-98 modulates macrophage polarization and suppresses the effects of tumor-associated macrophages on promoting invasion and epithelial-mesenchymal transition of hepatocellular carcinoma. Cancer Cell Int 18:95 (2018). PubMed: 29989015
- Zhang S et al. Tumor-associated macrophages promote tumor metastasis via the TGF-ß/SOX9 axis in non-small cell lung cancer. Oncotarget 8:99801-99815 (2017). IHC-Fr, IHC-P ; Human . PubMed: 29245941
- Peng QL et al. Elevated Serum Levels of Soluble CD163 in Polymyositis and Dermatomyositis: Associated with Macrophage Infiltration in Muscle Tissue. J Rheumatol 42:979-87 (2015). PubMed: 25877505
- Chamberlain CS et al. Interleukin-1 receptor antagonist modulates inflammation and scarring after ligament injury. Connect Tissue Res 55:177-86 (2014). PubMed: 24649870
- Chamberlain CS et al. Interleukin expression after injury and the effects of interleukin-1 receptor antagonist. PLoS One 8:e71631 (2013). IHC-Fr ; Rat . PubMed: 23936523