Recombinant
RabMAb

Recombinant Anti-CD163 antibody [SP96] - BSA and Azide free (ab236444)

Overview

  • Product name

    Anti-CD163 antibody [SP96] - BSA and Azide free
    See all CD163 primary antibodies
  • Description

    Rabbit monoclonal [SP96] to CD163 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide within Human CD163 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q86VB7

  • Positive control

    • IHC-P: Human bone marrow tissue.
  • General notes

    Ab236444 is the carrier-free version of ab100909. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab236444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Boil tissue section in EDTA pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate with primary antibody for 30 minutes at room temperature.

Target

  • Function

    Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
    After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
  • Tissue specificity

    Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
  • Sequence similarities

    Contains 9 SRCR domains.
  • Domain

    The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
  • Post-translational
    modifications

    A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
    Phosphorylated.
  • Cellular localization

    Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • C163A_HUMAN antibody
    • CD 163 antibody
    • CD163 antibody
    • CD163 antigen antibody
    • CD163 molecule antibody
    • Hemoglobin scavenger receptor antibody
    • M130 antibody
    • M130 antigen precursor antibody
    • Macrophage associated antigen antibody
    • MM130 antibody
    • OTTHUMP00000238617 antibody
    • OTTHUMP00000238618 antibody
    • OTTHUMP00000238619 antibody
    • OTTHUMP00000238620 antibody
    • SCARI1 antibody
    • Scavenger receptor cysteine rich type 1 protein M130 antibody
    • sCD163 antibody
    • Soluble CD163 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human esophagus tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab100909).

  • Formalin-fixed, paraffin-embedded human ovary tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab100909).

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab100909).

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab100909).

  • Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab100909).

  • Formalin-fixed, paraffin-embedded human lung tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab100909).

  • Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD163 using ab100909 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab100909).

References

ab236444 has not yet been referenced specifically in any publications.

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