Overview

  • Product name

    Anti-CD163L1 antibody [EPR6539]
    See all CD163L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6539] to CD163L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD163L1 aa 1400 to the C-terminus (intracellular). The exact sequence is proprietary.

  • Positive control

    • Human spleen and uterus tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6539
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/100 - 1/250.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Tissue specificity

      Isoform 1 is highly expressed in the spleen, lymph nodes, thymus, and fetal liver and weakly expressed in bone marrow and no expression was found in peripheral blood leukocytes. Isoform 1 expression is restricted to the monocyte and macrophage cell lines. Isoform 2 is only expressed in spleen.
    • Sequence similarities

      Contains 12 SRCR domains.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • B430307C05 antibody
      • C163B_HUMAN antibody
      • CD163 antigen B antibody
      • CD163 antigen-like 1 antibody
      • CD163 molecule-like 1 antibody
      • CD163b antibody
      • CD163b antigen antibody
      • CD163L1 antibody
      • CD164L1 antibody
      • E430002D04Rik antibody
      • M160 antibody
      • RGD1307378 antibody
      • Scart1 antibody
      • Scavenger receptor cysteine-rich type 1 protein M160 antibody
      see all

    Images

    • Immunohistochemical staining of paraffin embedded human stomach with purified ab126756 at a working dilution of 1/2000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemical staining of paraffin embedded human spleen with purified ab126756 at a working dilution of 1/2000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Unpurified ab126756, at 1/100 dilution, staining CD163L1 in paraffin-embedded Human spleen tissue by Immunohistochemsitry.

    • Unpurified ab126756, at 1/100 dilution, staining CD163L1 in paraffin-embedded Human uterus tissue by Immunohistochemistry.

    • Unpurified ab126756 showing positive staining in Normal tonsil macrophages tissue.

    • Unpurified ab126756 showing positive staining in Normal liver macrophages tissue.

    • Unpurified ab126756 showing positive staining in Normal lung macrophages tissue.

    • Unpurified ab126756 showing negative staining in Normal placenta tissue.

    • Unpurified ab126756 showing positive staining in Normal stomach macrophages tissue.

    References

    This product has been referenced in:

    • Nakazawa KR  et al. Accumulation and localization of macrophage phenotypes with human intervertebral disc degeneration. Spine J 18:343-356 (2018). Read more (PubMed: 29031872) »
    • Hu ZQ  et al. Overexpression of semaphorin 3A promotes tumor progression and predicts poor prognosis in hepatocellular carcinoma after curative resection. Oncotarget 7:51733-51746 (2016). IHC-P ; Human . Read more (PubMed: 27351132) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (metastatic melanoma)
    Permeabilization
    No
    Specification
    metastatic melanoma
    Blocking step
    (agent) for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Fixative
    Acetone

    Abcam user community

    Verified customer

    Submitted Sep 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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