Anti-CD166 antibody [3D9F1] (ab233750)
![Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)](https://www.abcam.com/ps/products/233/ab233750/Images/ab233750-307233-anti-cd166-antibody-3d9f1-flow-cytometry.jpg "Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)")
Key features and details
- Mouse monoclonal [3D9F1] to CD166
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD166 antibody [3D9F1]
See all CD166 primary antibodies -
Description
Mouse monoclonal [3D9F1] to CD166 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD166 aa 227-381. (Expressed in E.coli).
Database link: Q13740 -
Positive control
- WB: Human CD166 (aa 227-381) recombinant protein; CD166 (aa 227-381)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa and K562 cells. IHC-P: Human cervical cancer and bladder cancer tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3D9F1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab233750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 65 kDa. | |
| IHC-P | 1/200 - 1/1000. | |
| Flow Cyt | 1/200 - 1/400. |
Target
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Function
Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. -
Tissue specificity
Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Domain
The CD6 binding site is located in the N-terminal Ig-like domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 214 Human
- Omim: 601662 Human
- SwissProt: Q13740 Human
- Unigene: 591293 Human
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Alternative names
- Activated leukocyte cell adhesion molecule antibody
- ALCAM antibody
- ALCAM protein antibody
see all
Images
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Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD166 with ab233750 at 1/200 dilution (green) compared with a negative control (red).
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![Western blot - Anti-CD166 antibody [3D9F1] (ab233750)](https://www.abcam.com/ps/products/233/ab233750/Images/ab233750-307235-anti-cd166-antibody-3d9f1-western-blot.jpg)
Western blot - Anti-CD166 antibody [3D9F1] (ab233750)Anti-CD166 antibody [3D9F1] (ab233750) at 1/500 dilution + Human CD166 (aa 227-381) recombinant protein
Predicted band size: 65 kDaExpected MW is 47 kDa.
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![Western blot - Anti-CD166 antibody [3D9F1] (ab233750)](https://www.abcam.com/ps/products/233/ab233750/Images/ab233750-307234-anti-cd166-antibody-3d9f1-western-blot.jpg)
Western blot - Anti-CD166 antibody [3D9F1] (ab233750)All lanes : Anti-CD166 antibody [3D9F1] (ab233750) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CD166 (aa 227-381)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 65 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)](https://www.abcam.com/ps/products/233/ab233750/Images/ab233750-307232-anti-cd166-antibody-3d9f1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)Paraffin-embedded human cervical cancer tissue stained for CD166 using ab233750 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)](https://www.abcam.com/ps/products/233/ab233750/Images/ab233750-307231-anti-cd166-antibody-3d9f1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)Paraffin-embedded human bladder cancer tissue stained for CD166 using ab233750 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
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![Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)](https://www.abcam.com/ps/products/233/ab233750/Images/ab233750-307230-anti-cd166-antibody-3d9f1-flow-cytometry.jpg)
Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD166 with ab233750 at 1/200 dilution (green) compared with a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab233750 has not yet been referenced specifically in any publications.
