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    cd166-antibody-3d9f1-ab233750.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-CD166 antibody [3D9F1] (ab233750)

  • Datasheet
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Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)
  • Western blot - Anti-CD166 antibody [3D9F1] (ab233750)
  • Western blot - Anti-CD166 antibody [3D9F1] (ab233750)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)
  • Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)

Key features and details

  • Mouse monoclonal [3D9F1] to CD166
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-CD166 antibody [3D9F1]
    See all CD166 primary antibodies
  • Description

    Mouse monoclonal [3D9F1] to CD166
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD166 aa 227-381. (Expressed in E.coli).
    Database link: Q13740

  • Positive control

    • WB: Human CD166 (aa 227-381) recombinant protein; CD166 (aa 227-381)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa and K562 cells. IHC-P: Human cervical cancer and bladder cancer tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    3D9F1
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human CD166 protein (Fc Chimera) (ab191959)
  • Related Products

    • Recombinant Human CD166 protein (His tag) (ab220576)

Applications

Our Abpromise guarantee covers the use of ab233750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 65 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

Target

  • Function

    Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
  • Tissue specificity

    Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Domain

    The CD6 binding site is located in the N-terminal Ig-like domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q13740 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 214 Human
    • Omim: 601662 Human
    • SwissProt: Q13740 Human
    • Unigene: 591293 Human
    • Alternative names

      • Activated leukocyte cell adhesion molecule antibody
      • ALCAM antibody
      • ALCAM protein antibody
      • CD 166 antibody
      • CD166 antibody
      • CD166 antigen antibody
      • CD166_HUMAN antibody
      • FLJ3851 antibody
      • FLJ38514 antibody
      • MEMD antibody
      • MGC71733 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)
      Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)

      Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD166 with ab233750 at 1/200 dilution (green) compared with a negative control (red).

    • Western blot - Anti-CD166 antibody [3D9F1] (ab233750)
      Western blot - Anti-CD166 antibody [3D9F1] (ab233750)
      Anti-CD166 antibody [3D9F1] (ab233750) at 1/500 dilution + Human CD166 (aa 227-381) recombinant protein

      Predicted band size: 65 kDa



      Expected MW is 47 kDa.

    • Western blot - Anti-CD166 antibody [3D9F1] (ab233750)
      Western blot - Anti-CD166 antibody [3D9F1] (ab233750)
      All lanes : Anti-CD166 antibody [3D9F1] (ab233750) at 1/500 dilution

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
      Lane 2 : CD166 (aa 227-381)-hIgGFc transfected HEK-293 cell lysate

      Predicted band size: 65 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)

      Paraffin-embedded human cervical cancer tissue stained for CD166 using ab233750 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)

      Paraffin-embedded human bladder cancer tissue stained for CD166 using ab233750 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

    • Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)
      Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)

      Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD166 with ab233750 at 1/200 dilution (green) compared with a negative control (red).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab233750? Please let us know so that we can cite the reference in this datasheet.

    ab233750 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab233750.
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