Anti-CD166 antibody [3D9F1] (ab233750)

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Overview

  • Product name

    Anti-CD166 antibody [3D9F1]
    See all CD166 primary antibodies

  • Description

    Mouse monoclonal [3D9F1] to CD166

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human CD166 aa 227-381. (Expressed in E.coli).
    Database link: Q13740

  • Positive control

    • WB: Human CD166 (aa 227-381) recombinant protein; CD166 (aa 227-381)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa and K562 cells. IHC-P: Human cervical cancer and bladder cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab233750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 65 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

Target

  • Function

    Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.

  • Tissue specificity

    Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.

  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.

  • Domain

    The CD6 binding site is located in the N-terminal Ig-like domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activated leukocyte cell adhesion molecule antibody
      • ALCAM antibody
      • ALCAM protein antibody
      • CD 166 antibody
      • CD166 antibody
      • CD166 antigen antibody
      • CD166_HUMAN antibody
      • FLJ3851 antibody
      • FLJ38514 antibody
      • MEMD antibody
      • MGC71733 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)
      Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)

      Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD166 with ab233750 at 1/200 dilution (green) compared with a negative control (red).

    • Western blot - Anti-CD166 antibody [3D9F1] (ab233750)
      Western blot - Anti-CD166 antibody [3D9F1] (ab233750)
      Anti-CD166 antibody [3D9F1] (ab233750) at 1/500 dilution + Human CD166 (aa 227-381) recombinant protein

      Predicted band size: 65 kDa



      Expected MW is 47 kDa.

    • Western blot - Anti-CD166 antibody [3D9F1] (ab233750)
      Western blot - Anti-CD166 antibody [3D9F1] (ab233750)
      All lanes : Anti-CD166 antibody [3D9F1] (ab233750) at 1/500 dilution

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
      Lane 2 : CD166 (aa 227-381)-hIgGFc transfected HEK-293 cell lysate

      Predicted band size: 65 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)

      Paraffin-embedded human cervical cancer tissue stained for CD166 using ab233750 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)

      Paraffin-embedded human bladder cancer tissue stained for CD166 using ab233750 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

    • Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)
      Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)

      Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD166 with ab233750 at 1/200 dilution (green) compared with a negative control (red).

    References

    ab233750 has not yet been referenced specifically in any publications.

    Publishing research using ab233750? Please let us know so that we can cite the reference in this datasheet.

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