Recombinant
RabMAb

Recombinant Anti-CD166 antibody [EPR2759(2)] (HRP) (ab196846)

Overview

  • Product name

    Anti-CD166 antibody [EPR2759(2)] (HRP)
    See all CD166 primary antibodies
  • Description

    Rabbit monoclonal [EPR2759(2)] to CD166 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CD166. The exact sequence is proprietary.

  • Positive control

    • WB: SH-SY5Y whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 100-105 kDa (predicted molecular weight: 65 kDa).

Target

  • Function

    Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
  • Tissue specificity

    Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Domain

    The CD6 binding site is located in the N-terminal Ig-like domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activated leukocyte cell adhesion molecule antibody
    • ALCAM antibody
    • ALCAM protein antibody
    • CD 166 antibody
    • CD166 antibody
    • CD166 antigen antibody
    • CD166_HUMAN antibody
    • FLJ3851 antibody
    • FLJ38514 antibody
    • MEMD antibody
    • MGC71733 antibody
    see all

Images

  • Anti-CD166 antibody [EPR2759(2)] (HRP) (ab196846) at 1/1000 dilution + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Observed band size: 100-105 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196846 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab196846 has not yet been referenced specifically in any publications.

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