Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4054] to CD168
- Suitable for: WB, IP, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-CD168 antibody [EPR4054]
See all CD168 primary antibodies
DescriptionRabbit monoclonal [EPR4054] to CD168
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CD168 aa 1-100. The exact sequence is proprietary.
- Lysates of: MDA-MB-435, LnCaP, T47-D, RAW264.7, PC-12. Human testis tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.23 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab124729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 84 kDa.
For unpurified use at 1/1000 - 1/10000.
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
|IP||1/10 - 1/100.|
|IHC-P||1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/50 - 1/100.
Antigen retrieval is recommended
FunctionInvolved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.
Tissue specificityExpressed in breast cancer cell lines and in normal breast tissue.
Cellular localizationCell surface. Cytoplasm.
- Information by UniProt
- CD168 antibody
- CD168 antigen antibody
- HMMR antibody
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty
Lane 3: CD168 knockout HAP1 whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab124729 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab124729 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and CD168 knockout samples were subjected to SDS-PAGE. Ab124729 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CD168 with purified ab124729 at 1:250 dilution (7.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
ab124729 (purified) at 1:100 dilution (2ug) immunoprecipitating CD168 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate.
Lane 1 (input): MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab124729 & MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124729 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded human testis tissue using unpurified ab124729 at 1/50 - 1/100 dilution
All lanes : Anti-CD168 antibody [EPR4054] (ab124729) at 1/1000 dilution (purified)
Lane 1 : MDA-MB-435 (Human mammary gland ductal carcinoma melanocyte) whole cell lysates at 20 µg
Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates at 15 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDa
All lanes : Anti-CD168 antibody [EPR4054] (ab124729)
Lane 1 : MDA-MB-435 cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : T47-D cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 84 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124729 has been referenced in 5 publications.
- Chu TLH et al. Cell Cycle-Dependent Tumor Engraftment and Migration Are Enabled by Aurora-A. Mol Cancer Res 16:16-31 (2018). PubMed: 28993511
- Song JM et al. Triptolide suppresses the in vitro and in vivo growth of lung cancer cells by targeting hyaluronan-CD44/RHAMM signaling. Oncotarget 8:26927-26940 (2017). WB ; Human . PubMed: 28460475
- Connell M et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development. Elife 6:N/A (2017). PubMed: 28994651
- Buttermore ST et al. Increased RHAMM expression relates to ovarian cancer progression. J Ovarian Res 10:66 (2017). PubMed: 28954627
- Coulson-Thomas VJ et al. Umbilical Cord Mesenchymal Stem Cells Suppress Host Rejection: THE ROLE OF THE GLYCOCALYX. J Biol Chem 289:23465-81 (2014). ICC/IF ; Human . PubMed: 24986866