Validated using a knockout cell line
Recombinant
RabMAb

Anti-CD168 antibody [EPR4055] (ab108339)

Submit an Abreview Q&A (6)Specific References (3)

Overview

  • Product name
    Anti-CD168 antibody [EPR4055]
    See all CD168 primary antibodies
  • Description
    Rabbit monoclonal [EPR4055] to CD168
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human CD168.

  • Positive control
    • T47-D MCF-7, SKBR-3 and LnCaP cell lysate Paraffin-embedded human breast carcinoma tissue Paraffin-embedded human testis tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function
      Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.
    • Tissue specificity
      Expressed in breast cancer cell lines and in normal breast tissue.
    • Cellular localization
      Cell surface. Cytoplasm.
    • Information by UniProt
    • Database links
      • Alternative names
        • CD168 antibody
        • CD168 antigen antibody
        • HMMR antibody
        • HMMR_HUMAN antibody
        • Hyaluronan mediated motility receptor antibody
        • Hyaluronan-mediated motility receptor (RHAMM) antibody
        • IHABP antibody
        • Intracellular hyaluronic acid-binding protein antibody
        • MGC119494 antibody
        • MGC119495 antibody
        • OTTHUMP00000196920 antibody
        • Receptor for hyaluronan-mediated motility antibody
        • RHAMM antibody
        see all

      Images

      • Western blot - Anti-CD168 antibody [EPR4055] (ab108339)
        Western blot - Anti-CD168 antibody [EPR4055] (ab108339)

        Lane 1: Wild type HAP1 whole cell lysate (20 µg)
        Lane 2: Empty knockout  HAP1 whole cell lysate (20 µg)
        Lane 3: CD168 whole cell lysate (20 µg)              

        Lanes 1 - 3: Merged signal (red and green). Green - ab108339 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

        ab108339 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and Empty knockout samples were subjected to SDS-PAGE.  Ab108339 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        ab108339, at 1/100, staining CD168 in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
      • Western blot - Anti-CD168 antibody [EPR4055] (ab108339)
        Western blot - Anti-CD168 antibody [EPR4055] (ab108339)
        All lanes : Anti-CD168 antibody [EPR4055] (ab108339) at 1/1000 dilution

        Lane 1 : T47-D cell lysate
        Lane 2 : MCF-7 cell lysate
        Lane 3 : SKBR-3 cell lysate
        Lane 4 : LnCaP cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 84 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        ab108339, at 1/100, staining CD168 in paraffin-embedded human testis tissue by Immunohistochemistry.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        ab108339 showing positive staining in Normal thymus tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        ab108339 showing positive staining in Normal stomach tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        ab108339 showing negative staining in Normal brain tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        ab108339 showing positive staining in Colonic adenocarcinoma tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        ab108339 showing negative staining in Normal liver tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (ab108339)
        ab108339 showing positive staining in Normal tonsil tissue.

      References

      This product has been referenced in:
      • Wang K & Zhang T Prognostic significance of CD168 overexpression in colorectal cancer. Oncol Lett 12:2555-2559 (2016). IHC ; Human . Read more (PubMed: 27698827) »
      • Wang D  et al. Expression of the receptor for hyaluronic acid mediated motility (RHAMM) is associated with poor prognosis and metastasis in non-small cell lung carcinoma. Oncotarget 7:39957-39969 (2016). WB, IHC . Read more (PubMed: 27220886) »

      See all 3 Publications for this product

      Publishing research using ab108339? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      6 reviews or Q&A

      10
      Votes: Highest First

      Could you please provide me with the concentrations for items ab124729 (lot GR82264-8) and ab108339?

      For ab124729 lot GR82264-8, the concentration is 1.797 mg/ml.

      The current lot we have for sale for ab108339 is lot GR106937-4 and that is 0.193 mg/ml.

      The example on the Abcam website did say that fixation with paraformaldehyde was used but the cells were NOT permeabilized. So does this mean that the antibody recognizes denatured and not native protein or is the protein on the cell surface accessible...

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      There should not be concerns about permeabilizing cells because as soon as they are fixed with parafomraldehyde, they are essentially permeabilized. The CD168 antigen is a non-integral cell surface hyaluronan receptor that is readily accessable to the ...

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      Rabbit Mab, ab 108339 (clone EPR4055) – Do you have a protocol for using this antibody for flow cytometry ? I have tried this antibody using standard staining protocol and do not see any staining. Do the cells need to be fixed first (see 2D6 anti...

      Read More

      A link below is our Flow Cytometry protocol, which we use routinely for in-house testing.
      http://www.epitomics.com/products/support/fc
      Our protocol is for intracellular staining and we always fix the cells with 2% paraformaldehyde.

      Thank you for the information.




      It is really important forthe customerto know exactly which of the four known isoforms of RHAMM ab108339 recognizes.

      In the datasheet of the other antibody (ab67003) it says that it...

      Read More

      Thank you for your email.
      I can confirm that ab108339 (Anti-CD168 antibody) would recognize all 4 isoforms of CD168/RHAMM.
      If you need any further assistance in the future, please do not hesitate to contact me.

      Please send IHC-P protocol

      Thank you for contacting Abcam earlier today.

      Please find attached the IHC-P protocol for both ab108339 & ab124729.

      If there is anything else I can help you with, please let me know.

      Customer kindly asked
      - the immunogenic info of antibody.
      - Whether cells needs to permeabilized and fixed in FACS

      Thank you for contacting us.
      The target CD168 is a cell surface protein. There no need for cell permeabilization and fixation (do not wait longer than an hour). Cell fixatiion is advised if there is a wait longer than an hour between analysis. Avo...

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