Anti-CD168 antibody - N-terminal (ab185728)
Key features and details
- Rabbit polyclonal to CD168 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD168 antibody - N-terminal
See all CD168 primary antibodies -
Description
Rabbit polyclonal to CD168 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD168 aa 1-300 (N terminal).
Database link: O75330 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 84 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 84 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. -
Tissue specificity
Expressed in breast cancer cell lines and in normal breast tissue. -
Cellular localization
Cell surface. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3161 Human
- Omim: 600936 Human
- SwissProt: O75330 Human
- Unigene: 728200 Human
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Alternative names
- CD168 antibody
- CD168 antigen antibody
- HMMR antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185728. Blue DAPI for nuclear staining.
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All lanes : Anti-CD168 antibody - N-terminal (ab185728) at 1/500 dilution
Lane 1 : SW840 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : ES-2 cell lysate
Predicted band size: 84 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab185728 has been referenced in 3 publications.
- Leng Y et al. Hyaluronic acid, CD44 and RHAMM regulate myoblast behavior during embryogenesis. Matrix Biol 78-79:236-254 (2019). PubMed: 30130585
- Long KR et al. Extracellular Matrix Components HAPLN1, Lumican, and Collagen I Cause Hyaluronic Acid-Dependent Folding of the Developing Human Neocortex. Neuron 99:702-719.e6 (2018). PubMed: 30078576
- Oldenburg D et al. CD44 and RHAMM are essential for rapid growth of bladder cancer driven by loss of Glycogen Debranching Enzyme (AGL). BMC Cancer 16:713 (2016). WB, ICC/IF ; Human . PubMed: 27595989