Recombinant
RabMAb

Recombinant Anti-CD16a antibody [SP189] - C-terminal (ab227665)

Overview

  • Product name

    Anti-CD16a antibody [SP189] - C-terminal
    See all CD16a primary antibodies
  • Description

    Rabbit monoclonal [SP189] to CD16a - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD16a aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P08637

  • Positive control

    • IHC-P: Human lung, liver, thymus, spleen, placenta, B-cell lymphoma, cervical squamous cell carcinoma, colon carcinoma, HK lymphoma, stomach carcinoma, and liver hepatocellular carcinoma tissue; WB: K562 cell lysate; FC: Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Predicted molecular weight: 29 kDa.
IHC-P 1/100.

Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at
room temperature for 20 minutes. 

Flow Cyt 1/100.

Target

  • Function

    Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
  • Tissue specificity

    Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
  • Involvement in disease

    Immunodeficiency 20
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
    The soluble form is produced by a proteolytic cleavage.
  • Cellular localization

    Cell membrane. Secreted. Exists also as a soluble receptor.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD16a antibody
    • CD16a antigen antibody
    • Fc-gamma RIII antibody
    • Fc-gamma RIII-alpha antibody
    • Fc-gamma RIIIa antibody
    • FCG3A_HUMAN antibody
    • FCGR3A antibody
    • FcR-10 antibody
    • FcRIII antibody
    • FcRIIIa antibody
    • IgG Fc receptor III-2 antibody
    • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CD16a with ab227665 at 1/100 dilution (1.47 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD16a with ab227665 at 1/100 dilution (1.47 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD16a with ab227665 at 1/100 (green) compared with a rabbit isotype control (blue). 

  • Anti-CD16a antibody [SP189] - C-terminal (ab227665) at 1/100 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

    Developed using the ECL technique.

    Predicted band size: 29 kDa

  • Formalin-fixed, paraffin-embedded human lung tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

References

ab227665 has not yet been referenced specifically in any publications.

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