Recombinant Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP189] to CD16a - C-terminal, prediluted
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD16a antibody [SP189] - C-terminal, prediluted
See all CD16a primary antibodies -
Description
Rabbit monoclonal [SP189] to CD16a - C-terminal, prediluted -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung, liver, thymus, placenta, spleen, liver hepatocellular carcinoma, B-cell lymphoma, cervical squamous cell carcinoma, colon adenocarcinoma, stomach adenocarcinoma and Hodgkin's lymphoma tissues.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP189 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD16a antibody [SP189] - C-terminal (ab227665)
- Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)
- Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (ab246798)
- Alexa Fluor® 488 Anti-CD16 antibody [SP189] - C-terminal (ab309943)
- Alexa Fluor® 647 Anti-CD16 antibody [SP189] - C-terminal (ab310310)
- Alexa Fluor® 594 Anti-CD16 antibody [SP189] - C-terminal (ab310774)
- Alexa Fluor® 555 Anti-CD16 antibody [SP189] - C-terminal (ab312300)
- Alexa Fluor® 568 Anti-CD16 antibody [SP189] - C-terminal (ab312796)
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1.
Ready to use. Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature.
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Notes |
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IHC-P
1/1. Ready to use. Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature.
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Target
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Function
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. -
Tissue specificity
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. -
Involvement in disease
Immunodeficiency 20 -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
The soluble form is produced by a proteolytic cleavage. -
Cellular localization
Cell membrane. Secreted. Exists also as a soluble receptor. - Information by UniProt
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Database links
- Entrez Gene: 2214 Human
- SwissProt: P08637 Human
- Unigene: 372679 Human
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Alternative names
- CD16a antibody
- CD16a antigen antibody
- Fc-gamma RIII antibody
see all
Images
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Formalin-fixed, paraffin-embedded human lung tissue stained for CD16 using ab228278 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver tissue stained for CD16 using ab228278 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human thymus tissue stained for CD16 using ab228278 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for CD16 using ab228278 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human spleen tissue stained for CD16 using ab228278 in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228278 has not yet been referenced specifically in any publications.