Recombinant
RabMAb

Recombinant Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

Overview

  • Product name

    Anti-CD16a antibody [SP189] - C-terminal, prediluted
    See all CD16a primary antibodies
  • Description

    Rabbit monoclonal [SP189] to CD16a - C-terminal, prediluted
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD16a aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P08637

  • Positive control

    • IHC-P: Human lung, liver, thymus, placenta, spleen, liver hepatocellular carcinoma, B-cell lymphoma, cervical squamous cell carcinoma, colon adenocarcinoma, stomach adenocarcinoma and Hodgkin's lymphoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab228278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1.

Ready to use.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

 

Target

  • Function

    Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
  • Tissue specificity

    Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
  • Involvement in disease

    Immunodeficiency 20
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
    The soluble form is produced by a proteolytic cleavage.
  • Cellular localization

    Cell membrane. Secreted. Exists also as a soluble receptor.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD16a antibody
    • CD16a antigen antibody
    • Fc-gamma RIII antibody
    • Fc-gamma RIII-alpha antibody
    • Fc-gamma RIIIa antibody
    • FCG3A_HUMAN antibody
    • FCGR3A antibody
    • FcR-10 antibody
    • FcRIII antibody
    • FcRIIIa antibody
    • IgG Fc receptor III-2 antibody
    • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human lung tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

References

ab228278 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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