Anti-CD16b antibody [MM0272-5L11] (ab89207)
Key features and details
- Mouse monoclonal [MM0272-5L11] to CD16b
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2
Overview
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Product name
Anti-CD16b antibody [MM0272-5L11]
See all CD16b primary antibodies -
Description
Mouse monoclonal [MM0272-5L11] to CD16b -
Host species
Mouse -
Specificity
In recent direct ELISA testing, we found that this antibody cross-reacts ~50% with Human Fc gamma RIIIA/CD16a.
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Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human recombinant CD16b extracellular domain.
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Positive control
- WB: Human placenta and embryonic tissue lysates.
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General notes
Reconstituted antibody should be aliquoted and can be stored frozen at < -20°C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute the antibody with 200 µl sterile PBS to give a final concentration of 0.5mg/ml. -
Storage instructions
Shipped at 4°C. Store at -80°C. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography. -
Clonality
Monoclonal -
Clone number
MM0272-5L11 -
Isotype
IgG2 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB |
1/500 - 1/1000. Predicted molecular weight: 26 kDa.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/1000. Predicted molecular weight: 26 kDa. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 2215 Human
- Omim: 610665 Human
- SwissProt: O75015 Human
- Unigene: 372679 Human
- Unigene: 694258 Human
- Unigene: 736230 Human
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Alternative names
- CD16 antibody
- CD16b antibody
- CD16b antigen antibody
see all
Images
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Anti-CD16b antibody [MM0272-5L11] (ab89207) at 1/500 dilution + Human placenta tissue lysate
Predicted band size: 26 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted? -
Anti-CD16b antibody [MM0272-5L11] (ab89207) at 1/500 dilution + Human embryonic tissue lysate
Predicted band size: 26 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab89207 has not yet been referenced specifically in any publications.