Anti-CD16+CD32 antibody [2.4G2] (Phycoerythrin) (ab218781)

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Overview

  • Product name

    Anti-CD16+CD32 antibody [2.4G2] (Phycoerythrin)
    See all CD16+CD32 primary antibodies

  • Description

    Rat monoclonal [2.4G2] to CD16+CD32 (Phycoerythrin)

  • Host species

    Rat

  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • FACS: Mouse C57Bl/6 splenocytes.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.06% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation

  • Clonality

    Monoclonal

  • Clone number

    2.4G2

  • Isotype

    IgG2b

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab218781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance

    Function: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.

  • Cellular localization

    CD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.

  • Database links

    see all

  • Alternative names

    • CD16a antibody
    • CD16b antibody
    • CD32 antibody
    • CD32A antibody
    • CD32B antibody
    • CDw32 antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIIa receptor antibody
    • Fc fragment of IgG low affinity IIIb receptor antibody
    • Fc gamma receptor III A antibody
    • Fc gamma receptor IIIb antibody
    • Fc gamma RIIa antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIII beta antibody
    • Fc-gamma RII-a antibody
    • Fc-gamma-RIIa antibody
    • FCG2A_HUMAN antibody
    • FcGR antibody
    • FCGR2A antibody
    • FCGR2B antibody
    • FCGR3 antibody
    • FCGR3A antibody
    • FCGR3B antibody
    • FcRII-a antibody
    • FcRIII antibody
    • IgG Fc receptor II-a antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
    see all

Images

  • Flow Cytometry - Anti-CD16+CD32 antibody [2.4G2] (Phycoerythrin) (ab218781)
    Flow Cytometry - Anti-CD16+CD32 antibody [2.4G2] (Phycoerythrin) (ab218781)

    Flow cytrometrical analysis using C57Bl/6 splenocytes; stained with ab218781 at 0.125 µg dilution (solid line) or 0.125 µg PE Rat IgG2b isotype control (dashed line).

References

ab218781 has not yet been referenced specifically in any publications.

Publishing research using ab218781? Please let us know so that we can cite the reference in this datasheet.

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