Overview

  • Product name

  • Description

    Rabbit polyclonal to CD177
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Rat CD177 aa 540-590 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    VLSLRGGEIIS KLKVQGCMSQ PGCNLLNGTQ KIGPVDVSED CSPRSNSLTC


    Database link: D3ZCH9

  • Positive control

    • Rat colon tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.01% BSA, 50% Glycerol

    Aqueous buffered solution
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 87 kDa.
IHC-P 1/100 - 1/500.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200

Target

  • Tissue specificity

    Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).
  • Sequence similarities

    Contains 2 UPAR/Ly6 domains.
  • Post-translational
    modifications

    N-glycosylated.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD177 antibody
    • CD177 antigen antibody
    • CD177 molecule antibody
    • CD177_HUMAN antibody
    • HNA-2a antibody
    • HNA2A antibody
    • Human neutrophil alloantigen 2a antibody
    • NB1 antibody
    • NB1 glycoprotein antibody
    • NB1 GP antibody
    • Polycythemia rubra vera 1 antibody
    • Polycythemia rubra vera protein 1 antibody
    • PRV-1 antibody
    • PRV1 antibody
    see all

Images

  • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded rat colon labeling CD177 with ab203025 at 1/200 dilution followed by DAB staining.

References

This product has been referenced in:

  • Liu QY  et al. Inflammation responses in patients with pulmonary tuberculosis in an intensive care unit. Exp Ther Med 15:2719-2726 (2018). Read more (PubMed: 29456674) »
  • Jiang ZX  et al. Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis. Mol Med Rep 14:1083-90 (2016). IHC-P ; Mouse . Read more (PubMed: 27279017) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (neutrophils of tumor bearing mice)
Permeabilization
No
Gating Strategy
alive cells based on light scatter
Specification
neutrophils of tumor bearing mice
Preparation
Cell harvesting/tissue preparation method: cells were isolated using a sucrose density gradient
Sample buffer: staining was done in 0.5% BSA/PBS buffer
Fixation
none

Ms. Sandra Voels

Verified customer

Submitted Feb 06 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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