Recombinant Anti-CD177 antibody [EPR22813-205] (ab255296)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)](https://www.abcam.com/ps/products/255/ab255296/Images/ab255296-1-anti-cd177-antibody-epr22813-205-immunohistochemistry-bone-marrow-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22813-205] to CD177
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD177 antibody [EPR22813-205]
See all CD177 primary antibodies -
Description
Rabbit monoclonal [EPR22813-205] to CD177 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human bone marrow. Human placenta and spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22813-205 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 46 kDa.
|
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 46 kDa. |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). -
Sequence similarities
Contains 2 UPAR/Ly6 domains. -
Post-translational
modificationsN-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57126 Human
- Omim: 162860 Human
- SwissProt: Q8N6Q3 Human
- Unigene: 232165 Human
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Alternative names
- CD177 antibody
- CD177 antigen antibody
- CD177 molecule antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)
Immunohistochemical analysis of paraffin-embedded human bone marrow labeling CD177 with ab255296 at 1/2000 dilution (0.252 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human bone marrow (PMID: 22434870). The section was incubated with ab255296 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)](https://www.abcam.com/ps/products/255/ab255296/Images/ab255296-5-anti-cd177-antibody-epr22813-205-western-blot-human.jpg)
Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)All lanes : Anti-CD177 antibody [EPR22813-205] (ab255296) at 1/1000 dilution
Lane 1 : Human lung lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 46 kDaCD177 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 17580308).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 26 secs; Lane 2: 3 mins.
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![Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)](https://www.abcam.com/ps/products/255/ab255296/Images/ab255296-6-anti-cd177-antibody-epr22813-205-western-blot-liver-human.jpg)
Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)All lanes : Anti-CD177 antibody [EPR22813-205] (ab255296) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : U-937 (human histiocytic lymphoma monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 46 kDa
Exposure time: 3 minutesCD177 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 17580308). Negative control: U-937 (PMID: 17580308).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)](https://www.abcam.com/ps/products/255/ab255296/Images/ab255296-4-anti-cd177-antibody-epr22813-205-western-blot-cd177-protein-human.jpg)
Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)Anti-CD177 antibody [EPR22813-205] (ab255296) at 1/5000 dilution + Recombinant his-tagged human CD177 protein (aa22-407), 20 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Exposure time: 59 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)](https://www.abcam.com/ps/products/255/ab255296/Images/ab255296-3-anti-cd177-antibody-epr22813-205-immunohistochemistry-spleen-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD177 with ab255296 at 1/2000 dilution (0.252 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils in human spleen (PMID: 24562309) is observed. The section was incubated with ab255296 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)](https://www.abcam.com/ps/products/255/ab255296/Images/ab255296-2-anti-cd177-antibody-epr22813-205-immunohistochemistry-placenta-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling CD177 with ab255296 at 1/2000 dilution (0.252 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils in human placenta. The section was incubated with ab255296 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
-
![Anti-CD177 antibody [EPR22813-205] (ab255296)](https://www.abcam.com/ps/products/255/ab255296/Images/ab255296-7-benefits-of-recombinant-antibodies.png)
Anti-CD177 antibody [EPR22813-205] (ab255296)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab255296 has not yet been referenced specifically in any publications.
