Product nameAnti-CD18 antibody [2E6]
See all CD18 primary antibodies
DescriptionHamster monoclonal [2E6] to CD18
Tested applicationsSuitable for: Blocking, ICC, IP, Flow Cyt, Inhibition Assaymore details
Species reactivityReacts with: Mouse
- Recombinant Human CD18 protein (ab114283) can be used as a positive control in WB.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferConstituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab119347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
|Inhibition Assay||Use at an assay dependent concentration.|
FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Involvement in diseaseDefects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 VWFA domain.
modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
- Information by UniProt
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
Flow cytometry analysis of CD18 in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (ab119347) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.
This product has been referenced in:
- Tasaka S et al. Very late antigen-4 in CD18-independent neutrophil emigration during acute bacterial pneumonia in mice. Am J Respir Crit Care Med 166:53-60 (2002). Blocking ; Mouse . Read more (PubMed: 12091171) »
- Poon BY et al. alpha(4)-integrin mediates neutrophil-induced free radical injury to cardiac myocytes. J Cell Biol 152:857-66 (2001). Blocking ; Mouse . Read more (PubMed: 11238444) »