Anti-CD18 antibody [2E6] (ab119347)
Key features and details
- Hamster monoclonal [2E6] to CD18
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CD18 antibody [2E6]
See all CD18 primary antibodies -
Description
Hamster monoclonal [2E6] to CD18 -
Host species
Hamster -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length protein corresponding to Human CD18 aa 1-100. Native protein
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Positive control
- Flow Cyt: Recombinant Human CD18 protein (ab114283) can be used as a positive control in WB.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2E6 -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. -
Involvement in disease
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
Post-translational
modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16414 Mouse
- SwissProt: P11835 Mouse
- Unigene: 1137 Mouse
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Alternative names
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
Images
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Flow cytometry analysis of CD18 in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (ab119347) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.
Datasheets and documents
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Datasheet download
References (4)
ab119347 has been referenced in 4 publications.
- Tasaka S et al. Very late antigen-4 in CD18-independent neutrophil emigration during acute bacterial pneumonia in mice. Am J Respir Crit Care Med 166:53-60 (2002). Blocking ; Mouse . PubMed: 12091171
- Poon BY et al. alpha(4)-integrin mediates neutrophil-induced free radical injury to cardiac myocytes. J Cell Biol 152:857-66 (2001). Blocking ; Mouse . PubMed: 11238444
- Lawson JA et al. The hepatic inflammatory response after acetaminophen overdose: role of neutrophils. Toxicol Sci 54:509-16 (2000). Blocking ; Mouse . PubMed: 10774834
- Poon BY et al. Emigrated neutrophils regulate ventricular contractility via alpha4 integrin. Circ Res 84:1245-51 (1999). Blocking, Flow Cyt ; Mouse . PubMed: 10364561