Key features and details
- Goat polyclonal to CD18
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD18 antibody
See all CD18 primary antibodies
DescriptionGoat polyclonal to CD18
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Pig
- K562 and U937 cell lysates.
Reported variants represent identical protein: NP_001120963.1, NP_000202.2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab195994 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab195994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
1 hour primary incubation is recommended for this product.
FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Involvement in diseaseDefects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 VWFA domain.
modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
- Information by UniProt
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
All lanes : Anti-CD18 antibody (ab195994) at 0.3 µg/ml
Lane 1 : K562 cell lysate (in RIPA buffer)
Lane 2 : U937 cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
ab195994 has not yet been referenced specifically in any publications.