Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [C71/16] to CD18
- Suitable for: Flow Cyt
- Reacts with: Mouse
Product nameAnti-CD18 antibody [C71/16]
See all CD18 primary antibodies
DescriptionRat monoclonal [C71/16] to CD18
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus. This information is considered to be commercially sensitive.
- Flow Cyt: Mouse peripheral blood mononuclear cell (PBMC).
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityIon Exchange Chromatography
Our Abpromise guarantee covers the use of ab19580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1.003 µg/ml.|
FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Involvement in diseaseDefects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 VWFA domain.
modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
- Information by UniProt
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labelling CD18 with ab19580 at 1/1000 dilution (1.003µg/ml) (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labelling CD18 with ab19580 at 1/1000 dilution (1.003µg/ml) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab19580 has not yet been referenced specifically in any publications.