Overview

  • Product name
    Anti-CD18 antibody [EP1286Y]
    See all CD18 primary antibodies
  • Description
    Rabbit monoclonal [EP1286Y] to CD18
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CD18 aa 100-200 (N terminal). The exact sequence is proprietary.

  • Positive control
    • K562 cell membrane lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab53009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 95 kDa.
IP 1/50.

Target

  • Function
    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • Involvement in disease
    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications
    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 subunit beta antibody
    • Beta 2 (antigen CD18 (p95) antibody
    • CD 18 antibody
    • CD18 antibody
    • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
    • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
    • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
    • Complement component 3 receptor 3 and 4 subunit antibody
    • Complement receptor C3 beta subunit antibody
    • Complement receptor C3 subunit beta antibody
    • Integrin beta 2 antibody
    • Integrin beta chain beta 2 antibody
    • Integrin beta-2 (ITGB2) antibody
    • Integrin beta-2 antibody
    • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
    • ITB2_HUMAN antibody
    • ITGB2 antibody
    • LAD antibody
    • LCAMB antibody
    • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
    • Leukocyte cell adhesion molecule CD18 antibody
    • LFA 1 antibody
    • LFA1 antibody
    • Lymphocyte function associated antigen 1 (LFA1) antibody
    • Lymphocyte function associated antigen 1 antibody
    • MAC 1 antibody
    • MAC1 antibody
    • Macrophage antigen 1 (mac1) beta antibody
    • MF17 antibody
    • MFI7 antibody
    • OTTHUMP00000115278 antibody
    • OTTHUMP00000115279 antibody
    • OTTHUMP00000115280 antibody
    • OTTHUMP00000115281 antibody
    • OTTHUMP00000115282 antibody
    see all

Images

References

ab53009 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us and your interest in our products.

The anti-CD18 antibody [EP1286Y] (ab53009) has been used in Western blotting using human samples. As can be seen from the datasheet of this product, a strong band at ˜95 kDa was observed when using K562 lysate. This Western blot was performed under reducing, denaturing conditions and should therefore be suitable for your experiment. I have found reference to the protein being expressed in THP-1 cell line so this antibody should work well with your samples.


All of your antibodies are covered by the Abpromise guarantee. This means that if you have problems using the antibody in the applications or species listed on the datasheet (in this case Western blotting or immunoprecipitation with human samples) and you report these problems to us within 6 months of purchase, you would be eligible for a free of charge replacement, credit note or refund (whichever you prefer). More information on the Abpromise guarantee can be found form the following link:

www.abcam.com/abpromise

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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