Product nameAnti-CD18 antibody [M18/2]
See all CD18 primary antibodies
DescriptionRat monoclonal [M18/2] to CD18
Tested applicationsSuitable for: WB, IP, Functional Studies, Blocking, IHC-Fr, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse
Does not react with: Dog
Mouse cytotoxic T cell glycoproteins.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Purification notes> 95% (by SDS-PAGE)
Our Abpromise guarantee covers the use of ab119830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.|
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Involvement in diseaseDefects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 VWFA domain.
modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
- Information by UniProt
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
ICC/IF image of CD18 staining on RAW264.7 cells using ab119830 (1:300). The cells were fixed using formaldehyde and later permeabilized using 0.2% Triton X-100. The cells were blocked using 10% goat serum for 1 hour at 20°C. ab119830 was diluted to 1:300 and incubated with the cells for 1 hour at 20°C. The secondary antibody used was Anti-rat goat polyclonal conjugated to Alexa Fluor 647 (1:350).
This product has been referenced in:
- Liu YS et al. The pattern-recognition molecule mindin binds integrin Mac-1 to promote macrophage phagocytosis via Syk activation and NF-?B p65 translocation. J Cell Mol Med 23:3402-3416 (2019). Read more (PubMed: 30869196) »
- Mattei D et al. Maternal immune activation results in complex microglial transcriptome signature in the adult offspring that is reversed by minocycline treatment. Transl Psychiatry 7:e1120 (2017). IHC ; Mouse . Read more (PubMed: 28485733) »