Anti-CD18 antibody [M18/2] (ab119830)
Key features and details
- Rat monoclonal [M18/2] to CD18
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-CD18 antibody [M18/2]
See all CD18 primary antibodies -
Description
Rat monoclonal [M18/2] to CD18 -
Host species
Rat -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse
Does not react with: Dog -
Immunogen
Full length protein corresponding to Mouse CD18. Murine cytotoxic T cell glycoproteins
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Positive control
- Flow Cyt: Mouse splenocytes and myeloid cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
> 95% (by SDS-PAGE) -
Clonality
Monoclonal -
Clone number
M18/2 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 - 16 µg/ml.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use a concentration of 1 - 16 µg/ml. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. -
Involvement in disease
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
Post-translational
modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16414 Mouse
- SwissProt: P11835 Mouse
- Unigene: 1137 Mouse
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Alternative names
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
Images
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Flow cytometry analysis of mouse splenocytes labeling CD18 with ab119830 at 16 μg/mL.
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ICC/IF image of CD18 staining on RAW264.7 cells using ab119830 (1:300). The cells were fixed using formaldehyde and later permeabilized using 0.2% Triton X-100. The cells were blocked using 10% goat serum for 1 hour at 20°C. ab119830 was diluted to 1:300 and incubated with the cells for 1 hour at 20°C. The secondary antibody used was Anti-rat goat polyclonal conjugated to Alexa Fluor 647 (1:350).
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Flow Cytometry analysis of mouse myeloid cells labeling CD18 with ab119830 at 16 μg/mL (red-filled).
Mouse myeloid cells unstained by primary antibody (DAR APC, black-dashed).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab119830 has been referenced in 11 publications.
- Garcia-Arguinzonis M et al. Alternative C3 Complement System: Lipids and Atherosclerosis. Int J Mol Sci 22:N/A (2021). PubMed: 34066088
- Liang Y et al. PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production. Cell Death Differ 28:2728-2744 (2021). PubMed: 33934104
- Bain JM et al. Immune cells fold and damage fungal hyphae. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33876755
- Liu YS et al. The pattern-recognition molecule mindin binds integrin Mac-1 to promote macrophage phagocytosis via Syk activation and NF-?B p65 translocation. J Cell Mol Med 23:3402-3416 (2019). PubMed: 30869196
- Meng XL et al. Acute remote ischemic preconditioning alleviates free radical injury and inflammatory response in cerebral ischemia/reperfusion rats. Exp Ther Med 18:1953-1960 (2019). PubMed: 31410157