• Product name

    Anti-CD18 antibody [MEM-148]
    See all CD18 primary antibodies
  • Description

    Mouse monoclonal [MEM-148] to CD18
  • Host species

  • Specificity

    Recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1.
  • Tested applications

    Suitable for: Flow Cyt, IP, WB, Blocking, Functional Studiesmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Pig, Monkey
  • Immunogen

    Tissue/ cell preparation (Human). (Peripheral blood mononuclear cells).



Our Abpromise guarantee covers the use of ab8220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells.

The antibody is excellent marker of activated myeloid cells.





ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non-reducing conditions.
Blocking Use at an assay dependent concentration. PubMed: 23039186
Functional Studies Use at an assay dependent concentration. Induces homotypic cell aggregation and high affinity LFA1 conformation in resting leukocytes.


  • Function

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • Involvement in disease

    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational

    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • 95 subunit beta antibody
    • Beta 2 (antigen CD18 (p95) antibody
    • CD 18 antibody
    • CD18 antibody
    • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
    • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
    • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
    • Complement component 3 receptor 3 and 4 subunit antibody
    • Complement receptor C3 beta subunit antibody
    • Complement receptor C3 subunit beta antibody
    • Integrin beta 2 antibody
    • Integrin beta chain beta 2 antibody
    • Integrin beta-2 (ITGB2) antibody
    • Integrin beta-2 antibody
    • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
    • ITB2_HUMAN antibody
    • ITGB2 antibody
    • LAD antibody
    • LCAMB antibody
    • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
    • Leukocyte cell adhesion molecule CD18 antibody
    • LFA 1 antibody
    • LFA1 antibody
    • Lymphocyte function associated antigen 1 (LFA1) antibody
    • Lymphocyte function associated antigen 1 antibody
    • MAC 1 antibody
    • MAC1 antibody
    • Macrophage antigen 1 (mac1) beta antibody
    • MF17 antibody
    • MFI7 antibody
    • OTTHUMP00000115278 antibody
    • OTTHUMP00000115279 antibody
    • OTTHUMP00000115280 antibody
    • OTTHUMP00000115281 antibody
    • OTTHUMP00000115282 antibody
    see all


  • Human peripheral blood lymphocytes stained with ab8220 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8220, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • Transmigration of A375 Human melanoma cells, blocked using ab8220.

    Melanoma cells were labeled with 10 µM of lipophilic fluorescent dye for 20 minutes at 37°C. Cells were added to wells containing either 1 µg/ml of isotypic control, or 1 µg/ml of anti-CD11a (ab3981) or anti-CD18 (ab8220) antibodies. Melanoma cells were allowed to migrate at 37°C and 5% CO2 for 48 hours. Migration was observed under a fluorescence microscope.


This product has been referenced in:

  • Li D  et al. ß-1,3-Glucan/CR3/SYK pathway-dependent LC3B-II accumulation enhanced the fungicidal activity in human neutrophils. J Microbiol N/A:N/A (2019). Read more (PubMed: 30721460) »
  • Trentini A  et al. Hydroxyethyl Starch 130/0.4 Binds to Neutrophils Impairing Their Chemotaxis through a Mac-1 Dependent Interaction. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30769810) »
See all 9 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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There is a citation from the published methodology (Drbal et al. Blood 98(5), pp1561-1566, 2001): mAb used in paper: MEM-148 (ab8220) MEM-48 (standard anti CD18, #11-216) and other mAbs supplied by Abcam Cell were stained with mAbs (20 ug/ml) for 30 min on ice and washed in HBSS (Hanks balanced salt solution) containing 0.2% gelatin and 0.1% NaN3 without Ca++ and Mg++ followed by fluorescein labeled antimouse Ig. Propidium iodide (0.1 ug/ml) and LDS-751 (0.2 ug/ml) were added prior to measurement on FACS in a standard 3-color setup. At least 10000 viable nucleated cells (PI- , LDS-751+) were collected for each sample. Leucocyte populations were resolved on the basis of their scatter properties.

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It is not clear whether ab8220 recognizes the high-affinity (activated) conformation also on Mac-1, we have only indirect indications that it is the case but no definitive unambiguous data

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